{"product_id":"biolegend-847402","title":"Biolegend, 847402, Purified anti-ATG5 Antibody, 100μg","description":"\u003cp\u003eAutophagy protein 5 (ATG5) is involved in autophagic vesicle formation. ATG5 forms a complex with autophagy protein 12 (ATG12), which has E3 ubiquitin ligase activity, and is associated with autophagosome vesicle formation. ATG5 detaches from membranes immediately before or after autophagosome formation. ATG5 is also involved in mitochondrial quality control after oxidative damage, the innate anti-viral immune response, and apoptosis.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Human, Mouse, Rat, Drosophila\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: ATG5 peptide conjugated to KLH.\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: WB - Quality tested ICC, IHC-P - Verified Direct ELISA - Reported in the literature, not verified in house\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 1.0 µg\/mL in human, mouse, and rat and 1.0 - 10 µg\/mL in Drosophila For immunocytochemistry, a concentration range of 0.1 - 1.0 μg\/mL is recommended in human, mouse, rat and 1.0 - 5.0 μg\/mL in Drosophila. For immunohistochemical staining on formalin-fixed paraffin-embedded tissue sections, the suggested use of this reagent is 2.0 - 10 µg per mL. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: Reactivity to Drosophila was only verified with the purified format.\u003cbr\u003e\nRRID: AB_2734618 (BioLegend Cat. No. 847401) AB_2629668 (BioLegend Cat. No. 847402)\u003cbr\u003e\nStructure: Human ATG5 is a 275 amino acid protein with a molecular mass of 32 kD.\u003cbr\u003e\nDistribution: Tissue distribution: Ubiquitous. Cellular distribution: Vacuole, lysosome, and to a lesser extent mitochondria.\u003cbr\u003e\nFunction: ATG5 is involved in many cellular processes including autophagic vesicle formation, mitochondrial quality control after oxidative damage, innate anti-viral immune response, and apoptosis.\u003cbr\u003e\nInteraction: ATG12, LC3B, ATG16, TECPR1, Autophagy-linked FYVE protein.\u003cbr\u003e\nBiology Area: Cell Biology, Neurodegeneration, Neuroscience, Neuroscience Cell Markers, Protein Trafficking and Clearance\u003cbr\u003e\nMolecular Family: Autophagosome Markers\u003cbr\u003e\nAntigen References: 1. Uchiyama Y, et al. 2008. Histochem. Cell Biol. 129:407. PubMed 2. Wu H, et al. 2014. Int. J. Biol. Sci. 10:1072. PubMed 3. Subramani S, Malhotra V. 2013. EMBO Rep. 14(2):143. PubMed\u003cbr\u003e\nGene ID: 9474\u003cbr\u003e\nUniProt: View information about ATG5 on UniProt.org\u003cbr\u003e\nClone: 177.19\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Autophagy protein 5\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46860820742313,"sku":"847402","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-847402","provider":"Iright","version":"1.0","type":"link"}