{"product_id":"biolegend-852202","title":"Biolegend, 852202, Purified anti-TPP1 Antibody, 100μg","description":"\u003cp\u003eTPP1 is a member of the sedolisin family of serine proteases. The proenzyme is activated in the lysosome by auto-proteolysis where it cleaves tripeptides from the N-termini of substrates. Mutations of TPP1 are associated with late-infantile neuronal ceroid lipofuscinosis and Spinocerebellar Ataxia, Autosomal Recessive 7.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Recombinant mature human TPP1 expressed in E. coli.\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: WB - Quality testedIHC-P - Verified\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 5.0 µg per ml. For immunohistochemistry, a concentration range of 1.0 - 5.0 µg\/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: This clone does not recognize rodent (mouse or rat) TPP1.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Wujek P, et al. 2004. J. Biol. Chem. 279:12827-39.\u003cbr\u003e\nRRID: AB_2728589 (BioLegend Cat. No. 852201) AB_2728590 (BioLegend Cat. No. 852202)\u003cbr\u003e\nStructure: Human TPP1 is a 563 amino acid protein with a molecular mass of 61 kD. An isoform of 34.5 kD and 320 amino acids (244-563) is generated by alternative splicing.\u003cbr\u003e\nDistribution: Tissue sources: Ubiquitously expressed. Highest levels in heart, placenta, bone marrow, lung, pineal, and lymphocytes. Cellular distribution: Lysosome and extracellular.\u003cbr\u003e\nFunction: TPP1 cleaves tripeptides from the N-termini of substrates in lysosomes and has endopeptidase activity.\u003cbr\u003e\nInteraction: CLN5.\u003cbr\u003e\nBiology Area: Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation\u003cbr\u003e\nMolecular Family: Enzymes and Regulators, Proteases\u003cbr\u003e\nAntigen References: Getty AL, Pearce DA. 2011. Cell Mol. Life Sci. 68:453-74. Cárcel-Trullols J, et al. 2015. Biochim. Biophys. Acta. 2242-55. Kyttälä A, et al. 2006. Biochim Biophys Acta. 920-33.\u003cbr\u003e\nGene ID: 1200\u003cbr\u003e\nUniProt: View information about TPP1 on UniProt.org\u003cbr\u003e\nClone: 2E12\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Tripeptidyl Peptidase 1, Tripeptidyl Aminopeptidase, TPP-1, LPIC, Ceroid-Lipofuscinosis, Neuronal 2, Spinocerebellar Ataxia, Autosomal Recessive 7, Tripeptidyl-Peptidase 1, Tripeptidyl-Peptidase I, TPP I , SCAR7, GIG1\u003cbr\u003e\nIsotype: Mouse IgG2b, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46864367812777,"sku":"852202","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-852202","provider":"Iright","version":"1.0","type":"link"}