{"product_id":"biolegend-852801","title":"Biolegend, 852801, Purified anti-Apo E, 154-162 Antibody, 25μg","description":"\u003cp\u003eApolipoprotein E (Apo E) belongs to a class of apolipoproteins that mediate the binding, internalization, and catabolism of lipoprotein particles. Apo E is produced by the liver and macrophages in peripheral tissues where it regulates cholesterol metabolism. In the central nervous system, Apo E is primarily produced by astrocytes and plays an important role in transporting cholesterol to neurons via Apo E receptors. Apo E has three alleles (Apo E 2 , Apo E 3 and Apo E 4 ) that differ from each other by only one or two amino acids at positions 112 and 158. Apo E 4 is one of the largest known genetic risk factors for late-onset Alzheimer's disease. Furthermore, Apo E is associated with cardiovascular disease, Lipoprotein Glomerulopathy and Sea-Blue Histiocyte Disease.\u003cbr\u003e\n25μg\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: KLH conjugated Apo E peptide encompassing amino acids 154-162.\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/mL\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: WB - Quality tested Direct ELISA, IHC-P - Verified\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.2 - 1.0 µg per mL. For Direct ELISA applications, a concentration range of 0.004 - 0.4 µg\/mL is recommended. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1.0 - 5.0 µg\/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: This antibody cross-reacts with Apo E2, Apo E3, and Apo E4.\u003cbr\u003e\nRRID: AB_2728599 (BioLegend Cat. No. 852801) AB_2728600 (BioLegend Cat. No. 852802)\u003cbr\u003e\nStructure: Human Apo E is a 299 amino acid protein with a molecular mass of 32 kD, and exists as 3 major alleles, Apo E2, Apo E3, and Apo E4.\u003cbr\u003e\nDistribution: Tissue sources: Ubiquitously expressed. Highest levels in liver, brain, spleen, adrenal gland, and kidney. Cellular distribution: Plasma membrane, nucleus, endoplasmic reticulum, golgi apparatus, endosome, and extracellular.\u003cbr\u003e\nFunction: Apo E is involved in lipoprotein metabolism as well as the transport of fat-soluble vitamins, and cholesterol. Apo E also forms complexes with amyloid beta peptides leading to its uptake and degradation by microglia.\u003cbr\u003e\nInteraction: Tau, amyloid beta\u003cbr\u003e\nLigand\/Receptor: LDL receptor, SORL1.\u003cbr\u003e\nBiology Area: Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation\u003cbr\u003e\nMolecular Family: Apolipoproteins\u003cbr\u003e\nAntigen References: Mahley RW. 2016. Arterioscler Thromb Vasc Biol. 36: 1305-1315. Riedel BC, et al. 2016 J Steroid Biochem Mol Biol. 160:134-47. Tai LM, et al. 2016 Acta Neuropathol. 131(5):709-23. Kim J, et al. 2014. Mol. Cells. 37:767 Holtzman DM, et al. 2012. Cold Spring Harb. Perspect. Med. 2:a006312\u003cbr\u003e\nGene ID: 348\u003cbr\u003e\nUniProt: View information about Apo E 154-162 on UniProt.org\u003cbr\u003e\nClone: A17065A\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Apolipoprotein E, APO-E, Alzheimer Disease 2, LDLCQ5, APOE, LPG, AD2\u003cbr\u003e\nIsotype: Mouse IgG2b, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46864406053033,"sku":"852801","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-852801","provider":"Iright","version":"1.0","type":"link"}