{"product_id":"biolegend-865702","title":"Biolegend, 865702, Purified anti-CHIP Antibody, 100μg","description":"\u003cp\u003eCHIP is an E3 ubiquitin-protein ligase known to ubiquitinate  misfolded chaperone-bound substrates and target them for proteasomal degradation. Hsp70, Hsc70, and Hsp90  are three chaperone proteins with which CHIP interacts. CHIP mediates polyubiquitination of DNA polymerase beta at 'Lys-41', 'Lys-61' and 'Lys-81', thereby plays a role in base-excision repair. CHIP can also negatively regulate the suppressive function of regulatory T-cells during inflammation by mediating the ubiquitination and degradation of FOXP3 in a HSPA1A\/B-dependent manner. CHIP also negatively regulates TGF-beta signaling by modulating the basal levels of SMAD3 via ubiquitin-mediated degradation. Mutations in CHIP cause spinocerebellar ataxia type 16.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Human, Mouse, Rat\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: WB - Quality testedIHC-P - Verified\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 1.0 µg per ml. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 2.0 - 10 µg\/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nRRID: AB_2810779 (BioLegend Cat. No. 865701) AB_2810780 (BioLegend Cat. No. 865702)\u003cbr\u003e\nStructure: CHIP is a 303 amino acid protein with an apparent molecular mass of ~ 35 kD.\u003cbr\u003e\nDistribution: Tissue Distribution: Brain, heart, skeletal muscle, kidney, liver, and spleen Cellular Distribution: Cytosol, nucleus, and endoplasmic reticulum\u003cbr\u003e\nFunction: CHIP is involved in protein ubiquitination, protein degradation, and neurodegeneration.\u003cbr\u003e\nInteraction: Ubiquitin, ATXN3, EPHA2, SMAD3, UBE4B, VCP\/p97.\u003cbr\u003e\nCell Type: Glutamatergic Neurons, Mature Neurons, Neurons, Tregs\u003cbr\u003e\nBiology Area: Apoptosis\/Tumor Suppressors\/Cell Death, Cell Biology, Immunology, Neurodegeneration, Neuroscience, Signal Transduction, Ubiquitin\/Protein Degradation\u003cbr\u003e\nMolecular Family: Enzymes and Regulators, Heat Shock Proteins, Postsynaptic proteins, Phospho-Proteins, Protein Modifiers\u003cbr\u003e\nAntigen References: Zhan S, et al. 2017. Int Rev Immunol. 36(5):300. Cao Z, et al. 2016. Oncotarget. 7(20):29864.\u003cbr\u003e\nGene ID: 1027356424287155\u003cbr\u003e\nUniProt: View information about CHIP on UniProt.org\u003cbr\u003e\nClone: W17190B\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: CHIP, STIP1 Homology And U-Box Containing Protein 1, Carboxy Terminus Of Hsp70-Interacting Protein, RING-Type E3 Ubiquitin Transferase CHIP, E3 Ubiquitin-Protein Ligase CHIP, CLL-Associated Antigen KW-8, Antigen NY-CO-7, Heat Shock Protein A Binding\u003cbr\u003e\nIsotype: Rat IgG2b, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866315116713,"sku":"865702","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-865702","provider":"Iright","version":"1.0","type":"link"}