{"product_id":"biolegend-904402","title":"Biolegend, 904402, Purified anti-c-Myc Antibody (Previously Covance catalog# MMS-164P), 25microl","description":"\u003cp\u003ec-Myc truncations are often used as an epitope tag in order to simplify the labeling and detection of proteins. This antibody recognizes the c-Myc epitope tag and fused recombinant proteins allowing for specific detection in western blotting, immunoprecipitation, and immunostaining techniques.\u003cbr\u003e\n25microl\u003cbr\u003e\nVerified Reactivity: All Species\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nFormulation: Phosphate-buffered solution + 0.03% Thimerosal.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 1 mg\/mL\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.\u003cbr\u003e\nApplication: WB - Quality tested ICC, IHC-P, IP - Verified Purification - Reported in the literature, not verified in house\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg\/mL. For immunocytochemistry, a concentration range of 1.25 - 5.0 μg\/mL is recommended. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 2.5 - 5.0 µg\/mL is suggested. For immunoprecipitation, the suggested use of this reagent is 2.5 µg\/test. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: The 9E11 monoclonal antibody recognizes the sequence 408-420 AEEQKLISEEDL of human c-myc. This clone recognizes a non-specific band in BL21 E. coli lysates BioLegend offers two clones against c-Myc, 9E10 and 9E11. When both clones were tested in parallel for WB, ICC, and IP, each clone performed similarly, with 9E11 showing slightly better performance for each application.\u003cbr\u003e\nAdditional Product Notes: This antibody recognizes endogenous human c-Myc protein in IHC-P. Reactivity with endogenous c-Myc in other applications is not verified.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Halme A, et al. 2004. Cell. 116:405. Kolodziej P, Young R. 1991. Meth. Enzymol. 194:508. Locker AP, et al. 1989. Brit. J. Cancer. 60:669.\u003cbr\u003e\nProduct Citations: Sims S, et al. 2021. Adv Virol. 2021:5569844. PubMed Yi B, et al. 2022. Oncogene. 41:268. PubMed Wu Y, et al. 2016. Nat Struct Mol Biol. 10.1038\/nsmb.3284. PubMed\u003cbr\u003e\nRRID: AB_2565039 (BioLegend Cat. No. 904401)\u003cbr\u003e\nBiology Area: Cell Biology, Protein Purification, Protein Synthesis\u003cbr\u003e\nGene ID: 4609\u003cbr\u003e\nUniProt: View information about c-Myc on UniProt.org\u003cbr\u003e\nClone: 9E11\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: myc tag, c-Myc tag, cMyc tag, Myc epitope tag\u003cbr\u003e\nPreviously: Covance Catalog# MMS-164P\u003cbr\u003e\nIsotype: Mouse IgG2a, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862507442345,"sku":"904402","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-904402","provider":"Iright","version":"1.0","type":"link"}