{"product_id":"biolegend-914211","title":"Biolegend, 914211, Alexa Fluor® 647 anti-Pan-Cytokeratin Antibody, 25μg","description":"\u003cp\u003eAE-1 immunoreacts with an antigenic determinant present on most of the subfamily A cytokeratins, including cytokeratins with molecular weights of 56.5, 50, 48 and 40 kD. AE-3 reacts with an antigenic determinant shared by the subfamily B cytokeratins including cytokeratins with molecular weights of of 64, 59, 58, 56, and 52 kD.\u003cbr\u003e\n25μg\u003cbr\u003e\nVerified Reactivity: Human, Rat\u003cbr\u003e\nReported Reactivity: Dog, Non-Human Primate\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: This antibody was developed using human epidermal keratins.\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.\u003cbr\u003e\nConcentration: 0.5 mg\/mL\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: IHC-P - Quality tested ICC, ICFC - Verified\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1 - 10 µg\/mL is suggested. For immunocytochemistry, a concentration range of 1 - 10 μg\/mL is recommended. For flow cytometric staining using our Cyto-Fast™ Fix\/Perm Buffer Set, the suggested use of this reagent is ≤ 0.06 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm \/ 635 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses\u003cbr\u003e\nExcitation Laser: Red Laser (633 nm)\u003cbr\u003e\nAdditional Product Notes: For use of this antibody (clone AE-1\/AE-3) in immunocytochemistry (ICC), we recommend fixation with Fixation Buffer (Cat. No. 420801) followed by permeabilization with either 0.5% Triton-X or 100% ice-cold methanol, or fixation\/permeabilization with 100% ice-cold methanol alone. For use of this antibody (clone AE-1\/AE-3) in intracellular flow cytometry (ICFC), we recommend fixation\/permeabilization using Cyto-FastTM Fix\/Perm Buffer Set (Cat. No. 426803). For use of this antibody (clone AE-1\/AE-3) in immunohistochemistry (IHC), we recommend antigen retrieval with either Sodium Citrate H.I.E.R. (Cat. No. 928502) or Tris-EDTA pH 9.0 Antigen Retrieval Buffer (Cat. No. 422703).\u003cbr\u003e\nRRID: AB_3675197 (BioLegend Cat. No. 914211) AB_3675197 (BioLegend Cat. No. 914212)\u003cbr\u003e\nDistribution: Cytoplasm, cell membrane\u003cbr\u003e\nFunction: Cytoskeleton, cell motility and structure\u003cbr\u003e\nBiology Area: Cell Biology, Cell Motility\/Cytoskeleton\/Structure, Neuroscience, Neuroscience Cell Markers\u003cbr\u003e\nMolecular Family: Intermediate Filaments\u003cbr\u003e\nAntigen References: Metcalf KJ, et al. 2022. Matrix Biol Plus. 14:100105. Jiang S, et al. 2022. Immunity. 55:1118. Tiago M, et al. 2020. Br J Cancer. 122:789. Kou R, et al. 2018. Oncol Rep. 7:2013. Xie S, et al. 2020. Ann Transl Med. 1.384027778. Chang TH, et al. 2020. Cell Death Dis. 11:338. Kennedy-Darling J, et al. 2021. Eur J Immunol. 51:1262. Cai S, et al. 2022. iScience. 25:104980.\u003cbr\u003e\nGene ID: NA\u003cbr\u003e\nUniProt: View information about Pan-Cytokeratin on UniProt.org\u003cbr\u003e\nClone: AE-1\/AE-3\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Pan-Cytokeratin (AE-1\/AE-3), AE1\/AE3\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866079613097,"sku":"914211","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-914211","provider":"Iright","version":"1.0","type":"link"}