{"product_id":"biolegend-934103","title":"Biolegend, 934103, PE anti-HMOX1 Antibody, 25tests","description":"\u003cp\u003eHMOX1 is a member of the heme oxygenase family. Unlike its constitutive counterpart, it is an inducible enzyme triggered by external stimuli such as nitric oxide, growth factors, heavy metals, UV radiation, phorbol esters, inflammatory cytokines, heme, etc. Transcription of HMOX1 in tissue has been shown to be a product of the nrf2\/ARE pathway where an increase in its protein levels results in heme degradation. The tissue protective effects of HMOX1 are a result of the bioactive products produced after heme degradation: iron ions, carbon monoxide, and biliverdin which is converted to bilirubin via biliverdin reductase.\u003cbr\u003e\n25tests\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: ICFC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)Green Laser (532 nm)\/Yellow-Green Laser (561 nm)\u003cbr\u003e\nApplication Notes: Clone was tested for ICC using three fix\/perm methods (PFA\/Triton-X, PFA\/MeOH, MeOH only), the best method was the use of PFA\/Triton X-100. In the CoCl2 treated cells for ICC, staining was seen in less than 5% of cells. Clone was tested for ICFC using cytokine fix\/perm method only (based on ICC results).\u003cbr\u003e\nRRID: AB_2941636 (BioLegend Cat. No. 934103) AB_2941636 (BioLegend Cat. No. 934104)\u003cbr\u003e\nDistribution: Cytosol\/Endoplasmic reticulum\u003cbr\u003e\nBiology Area: Cell Biology\u003cbr\u003e\nMolecular Family: Enzymes and Regulators\u003cbr\u003e\nAntigen References: 1. Yang B, et al. 2018. Toxicol Appl Pharmacol. 355:189-197 2. Chora A, et al. 2007. J Clin Invest. 117(2):438-47 3. Harding H, et al. 2003. Mol Cell. 11(3):619-33.\u003cbr\u003e\nGene ID: 3162\u003cbr\u003e\nUniProt: View information about HMOX1 on UniProt.org\u003cbr\u003e\nClone: W19398C\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Heme Oxygenase 1, HO-1, Heat Shock Protein, 32-KD\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003cbr\u003e\nQ: What type of PE do you use in your conjugates?\u003cbr\u003e\nA: We use R-PE in our conjugates.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866416631977,"sku":"934103","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-934103","provider":"Iright","version":"1.0","type":"link"}