{"product_id":"biolegend-934105","title":"Biolegend, 934105, Alexa Fluor® 647 anti-HMOX1 Antibody, 25tests","description":"\u003cp\u003eHMOX1 is a member of the heme oxygenase family. Unlike its constitutive counterpart, it is an inducible enzyme triggered by external stimuli such as nitric oxide, growth factors, heavy metals, UV radiation, phorbol esters, inflammatory cytokines, heme, etc. Transcription of HMOX1 in tissue has been shown to be a product of the nrf2\/ARE pathway where an increase in its protein levels results in heme degradation. The tissue protective effects of HMOX1 are a result of the bioactive products produced after heme degradation: iron ions, carbon monoxide, and biliverdin which is converted to bilirubin via biliverdin reductase.\u003cbr\u003e\n25tests\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nFormulation: µg size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide test sizes: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.\u003cbr\u003e\nConcentration: µg sizes: 0.5 mg\/mLtest sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: ICFC (test sizes) - Quality tested ICC (µg size) - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of the test-sized antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining using the test sizes, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. Each lot of the µg-sized antibody is quality control tested by immunocytochemistry. For immunocytochemistry using the ug size, a concentration range of 1.25 - 5.0 μg\/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm \/ 635 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses\u003cbr\u003e\nExcitation Laser: Red Laser (633 nm)\u003cbr\u003e\nApplication Notes: Clone was tested for ICC using three fix\/perm methods (PFA\/Triton-X, PFA\/MeOH, MeOH only), the best method was the use of PFA\/Triton X-100. In the CoCl2 treated cells for ICC, staining was seen in less than 5% of cells. Clone was tested for ICFC using cytokine fix\/perm method only (based on ICC results).\u003cbr\u003e\nRRID: AB_2941637 (BioLegend Cat. No. 934105) AB_2941637 (BioLegend Cat. No. 934106) AB_2941637 (BioLegend Cat. No. 934108)\u003cbr\u003e\nDistribution: Cytosol\/Endoplasmic reticulum\u003cbr\u003e\nBiology Area: Cell Biology\u003cbr\u003e\nMolecular Family: Enzymes and Regulators\u003cbr\u003e\nAntigen References: 1. Yang B, et al. 2018. Toxicol Appl Pharmacol. 355:189-197 2. Chora A, et al. 2007. J Clin Invest. 117(2):438-47 3. Harding H, et al. 2003. Mol Cell. 11(3):619-33.\u003cbr\u003e\nGene ID: 3162\u003cbr\u003e\nUniProt: View information about HMOX1 on UniProt.org\u003cbr\u003e\nClone: W19398C\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Heme Oxygenase 1, HO-1, Heat Shock Protein, 32-KD\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866416369833,"sku":"934105","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-934105","provider":"Iright","version":"1.0","type":"link"}