{"product_id":"biolegend-935601","title":"Biolegend, 935601, Purified anti-GIT1 Antibody, 25μg","description":"\u003cp\u003eGIT1 is a G protein-coupled receptor kinase-interacting protein which is a component of the PIX\/Rac\/PAK pathway that is involved in cellular development. In this pathway, Rac activates PAK which leads to the phosphorylation of PIX and GIT1. The downstream result to these interactions leads to the formation of focal adhesion complexes in the periphery of the cell membrane with various proteins including FAK and paxillin. GIT1's various protein-protein interactions in the focal complex lead to downstream cellular events such as migration, morphogenesis and neuronal growth (spine\/synapse formation).\u003cbr\u003e\n25μg\u003cbr\u003e\nVerified Reactivity: Human, Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Partial recombinant human GIT1 protein of approximately 250 amino acids surrounding amino acid 500\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/mL\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: WB - Quality testedICC, IP - Verified\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.5 - 1.0 µg\/mL. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg\/mL is recommended. For immunoprecipitation, the suggested use of this reagent is 2.0 µg\/test. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: Non-specific bands present in HeLa lysates.Triton X-100 is the best ICC permeabilization method for this antibody.\u003cbr\u003e\nRRID: AB_2832898 (BioLegend Cat. No. 935601) AB_2832899 (BioLegend Cat. No. 935602)\u003cbr\u003e\nStructure: GIT1 is a 761 amino acid protein with a predicted molecular weight of 84 kD.\u003cbr\u003e\nDistribution: Cytosol and focal adhesion sites\u003cbr\u003e\nFunction: GTPase activation\u003cbr\u003e\nBiology Area: Cell Biology, Cell Motility\/Cytoskeleton\/Structure, Protein Trafficking and Clearance\u003cbr\u003e\nMolecular Family: GTPases, Scaffold Proteins\u003cbr\u003e\nAntigen References: Zhang H, et al. 2005. J. Neurosci. 25:3379. Zhao ZS, et al. 2000. Mol. Cell. Biol. 20:6354. Goehler H, et al. 2004. Mol. Cell. 15:853.\u003cbr\u003e\nGene ID: 28964\u003cbr\u003e\nUniProt: View information about GIT1 on UniProt.org\u003cbr\u003e\nClone: W18253B\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: G Protein-Coupled Receptor Kinase Interacting, ArfGAP 1, GRK-Interacting Protein 1, ARF GAP GIT1, CAT1, Cool-associated and tyrosine phosphorylated protein 1, ARF GTPase-activating protein GIT1\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866337726633,"sku":"935601","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-935601","provider":"Iright","version":"1.0","type":"link"}