{"product_id":"biolegend-940401","title":"Biolegend, 940401, Purified anti-SARS-CoV-2 S Protein S1 Recombinant Antibody, 25μg","description":"\u003cp\u003eSevere acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a single- stranded RNA virus that belongs in a family of viruses known as coronaviruses.   SARS-CoV-2 infection, known as COVID-19, was declared a pandemic by WHO on March 11, 2020 and among other symptoms leads to respiratory infection, pulmonary failure which can be fatal. SARS-CoV-2 is structurally composed of 4 main proteins (e.g. spike glycoprotein, envelope glycoprotein, membrane glycoprotein and nucleocapsid protein) and several accessory proteins. The spike glycoprotein (S) is a transmembrane molecule that forms homotrimers on the surface of the virus and facilitates virulence through binding to angiotensin-converting enzyme 2 (ACE2) expressed on host cells. This protein is composed of two subunits S1 and S2. S1 subunit is responsible for binding to the host cell via ACE-2 receptor and S2 for fusion with the host cell.  This antibody is specific for the S1 subunit of SARS-CoV-2 and was derived, sequenced and expressed from patients that had recovered from COVID-19 infection.\u003cbr\u003e\n25μg\u003cbr\u003e\nVerified Reactivity: SARS-CoV-2\u003cbr\u003e\nAntibody Type: Recombinant\u003cbr\u003e\nHost Species: Human\u003cbr\u003e\nImmunogen: SARS-CoV-2\u003cbr\u003e\nFormulation: 140 mM Hepes, pH 7.5, 70 mM NaCl, 32 mM NaOAc, 0.035% sodium azide\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 1.0 mg\/mL\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Prior to use, quick spin the antibody vial to ensure recovery of maximum volume. Antibody can be stored in single use aliquots in -20°C for 2 years. Avoid repeated freeze and thaws.\u003cbr\u003e\nApplication: WB - Quality tested Direct ELISA - Verified\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.125 - 1.0 µg\/mL. Recommended usage concentration for direct ELISA application is 93.6 ng\/mL. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: Clone name AM004414 is also referred to as clone 414-4.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): 1. Wan J. et al. 2020. Cell Rep. 32(3):107918\u003cbr\u003e\nRRID: AB_2888747 (BioLegend Cat. No. 940401) AB_2888747 (BioLegend Cat. No. 940402)\u003cbr\u003e\nStructure: Spike glycoprotein is a homotrimer. Each monomer consists of 1,273 a.a with a molecular weight of approximately 141 kD and consists of S1 and S2 subunits. S1 subunit is 673 a.a. long.\u003cbr\u003e\nDistribution: Viral envelope protein, host cell membrane, host cell endoplasmic reticulum-Golgi intermediate compartment membrane\u003cbr\u003e\nFunction: Host cell surface receptor binding\u003cbr\u003e\nInteraction: ACE2\u003cbr\u003e\nLigand\/Receptor: ACE2\u003cbr\u003e\nBiology Area: COVID-19\u003cbr\u003e\nAntigen References: 1. Walls A.C. et al. 2020. Cell 181(2):281-292 2. Yan R. et al. 2020 Science 367 (6485):1444-1448 3. Wrapp D. et al. 2020 Science 367 (6483):1260-126 4. Shang J.et al. 2020 PNAS 117(21):11727-11734\u003cbr\u003e\nGene ID: 43740568\u003cbr\u003e\nUniProt: View information about Viral Protein on UniProt.org\u003cbr\u003e\nClone: AM004414\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Spike glycoprotein, S glycoprotein (E2)\u003cbr\u003e\nIsotype: Human IgG1, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862539554985,"sku":"940401","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-940401","provider":"Iright","version":"1.0","type":"link"}