{"product_id":"biolegend-945201","title":"Biolegend, 945201, Purified anti-Merlin (NF2) Antibody, 25μg","description":"\u003cp\u003eMoesin-ezrin-radixin-like protein (Merlin) is a tumor suppressor protein encoded by the Neurofibromin 2 (NF2) gene. It functions as a scaffold linking the plasma membrane and the cytoskeleton, and plays a crucial role in regulating cell polarization and morphology. Merlin functions as a major upstream activator of the Hippo signaling pathway, where it promotes Lats1\/2 degradation, leading to increased nuclear localization of the YAP\/TAZ transcription factors. Mutations and deletions within the NF2 gene cause neurofibromatosis type 2, a tumorigenic disease of the brain.\u003cbr\u003e\n25μg\u003cbr\u003e\nVerified Reactivity: Human, Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Synthetic peptide corresponding to human Merlin.\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/mL\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: WB - Quality tested IP - Verified\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.5 - 2.0 µg\/mL. For immunoprecipitation, the suggested use of this reagent is 2.5 µg\/test. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: This clone was tested for ICC using 4% PFA-fixed HeLa cells permeabilized with either methanol or Triton X-100, and methanol-fixed and permeabilized HeLa cells. None of the methods were compatible with Merlin staining. This clone is not predicted to cross-react with other Merlin homologs due to poor sequence conservation between the immunizing peptide and the corresponding region in each homolog.\u003cbr\u003e\nRRID: AB_2892510 (BioLegend Cat. No. 945201) AB_2892510 (BioLegend Cat. No. 945202)\u003cbr\u003e\nStructure: Merlin is a 595 amino acid protein with a predicted molecular weight of 70 kD.\u003cbr\u003e\nDistribution: Ubiquitously expressed\/Cytosol\u003cbr\u003e\nFunction: Tumor suppression, apoptosis\u003cbr\u003e\nBiology Area: Apoptosis\/Tumor Suppressors\/Cell Death, Cell Adhesion, Cell Biology, Cell Motility\/Cytoskeleton\/Structure\u003cbr\u003e\nAntigen References: Li W, et al. 2010. Cell. 140:477-490 Petrilli A, et al. 2016. Oncogene. 35:537-548. White S, et al. 2019. Dev Cell. 49:425-443.E9.\u003cbr\u003e\nGene ID: 4771\u003cbr\u003e\nUniProt: View information about Merlin on UniProt.org\u003cbr\u003e\nClone: W19231A\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Neurofibromin 2, NF2, Merlin, Schwannomin, SCH, Neurofibromin 2 (Bilateral Acoustic Neuroma), Moesin-Ezrin-Radixin-Like Protein, Moesin-Ezrin-Radixin Like, Schwannomerlin, BANF, ACN\u003cbr\u003e\nIsotype: Rat IgG2b, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866413224105,"sku":"945201","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-945201","provider":"Iright","version":"1.0","type":"link"}