{"product_id":"biolegend-946605","title":"Biolegend, 946605, PE anti-LAT Phospho (Tyr171) Antibody, 25tests","description":"\u003cp\u003eLAT, or Linker for Activation of T cells, is a transmembrane protein expressed mainly in T cells and a limited number of other immune cells such as natural killer cells, mast cells, and immature B cells. As an adaptor protein, phosphorylated LAT facilitates the recruitment of other signaling proteins and functions as a nucleating site for multiprotein signaling complexes. These signaling complexes can propagate TCR, or T Cell antigen Receptors, and activate downstream effectors, which trigger T cell proliferation or cytokine expression.  LAT (Tyr171) is one of the nine tyrosines in LAT conserved between human, mice, and rat. LAT (Tyr171) is necessary for the activation of PI 3-kinase, a protein that regulates the recruitment of other proteins that facilitate in T-cell receptor signal transduction such as PLC-gamma1 and Sos1. When LAT (Tyr171) and (Tyr191) is phosphorylated by ZAP-70, Gads can bind directly to LAT (Tyr171) and (Tyr191) and mediate the recruitment of SLP-76.\u003cbr\u003e\n25tests\u003cbr\u003e\nVerified Reactivity: Human, Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Synthetic peptide corresponding to human LAT phosphorylated at Tyr 171\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: ICFC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)Green Laser (532 nm)\/Yellow-Green Laser (561 nm)\u003cbr\u003e\nApplication Notes: When this clone is used in WB at a dilution lower than 1:1000 it may show non-specific bands at 50 kD and above. This clone has been tested by ICC on H2O2 treated and untreated Jurkat, with three fix and permeabilization methods (100% methanol, 4% PFA plus methanol and 4% PFA plus Triton X-100). All three methods can be used for ICC staining, but the PFA plus Triton X-100 fixation\/permeabilization method produced the best staining. This clone has only been tested on True-PhosTM perm buffer (Cat. No. 425401) by ICFC\u003cbr\u003e\nRRID: AB_2936765 (BioLegend Cat. No. 946605) AB_2936765 (BioLegend Cat. No. 946606)\u003cbr\u003e\nStructure: LAT is a 262 amino acid protein with a predicted molecular weight of 36-38 kD.\u003cbr\u003e\nDistribution: Plasma membrane\/T Cells\u003cbr\u003e\nFunction: Plasma membrane\/T Cells\u003cbr\u003e\nCell Type: Mast cells, NK cells, T cells\u003cbr\u003e\nBiology Area: Cell Biology, Immunology, Signal Transduction\u003cbr\u003e\nMolecular Family: Phospho-Proteins\u003cbr\u003e\nAntigen References: Balagopalan L. et al., 2010, Cold Spring Harb Perspect Biol., 2(8): a005512 Balagopalan L. et al., 2020, PLoS One, 15(2): e0229036 Lin J. and Weiss A., 2001, J Biol Chem., 276(31): 29588-95 Paz et. al., 2001, Biochem J., 356, 461-71 Zhang W. et al., 2000, J Biol Chem., 275(30): 23355-61\u003cbr\u003e\nGene ID: 27040\u003cbr\u003e\nUniProt: View information about LAT Phospho Tyr171 on UniProt.org\u003cbr\u003e\nClone: A20005D\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Linker For Activation Of T Cells; P36-38\u003cbr\u003e\nIsotype: Mouse IgG2b, κ\u003cbr\u003e\nQ: What type of PE do you use in your conjugates?\u003cbr\u003e\nA: We use R-PE in our conjugates.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866068537513,"sku":"946605","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-946605","provider":"Iright","version":"1.0","type":"link"}