{"product_id":"biolegend-947809","title":"Biolegend, 947809, PE\/Cyanine7 anti-human CD273 (B7-DC, PD-L2) Antibody, 25tests","description":"\u003cp\u003ePD-L2 is a type I transmembrane protein of 273 amino acids, and it is a member of the B7 family. Human PD-L2 has 70% amino acid identity to its mouse orthologue. PD-L2 is the second ligand for PD-1, and its binding to PD-1 leads to the inhibition of T cell receptor–mediated lymphocyte proliferation and cytokine secretion. PD-L2\/PD-1 interaction suppresses immune responses against autoantigens and tumors and plays an important role in the maintenance of peripheral immune tolerance. Antibody blocking of PD-L2 on dendritic cells results in enhanced T cell proliferation and cytokine production. Interaction of PD-1 by PD-L1-Ig or PD-L2-Ig fusion protein has an inhibitory effect on proliferation and cytokine production during anti-CD3-mediated stimulation. PD-L2 is expressed in biopsies from human asthmatics and lungs of aeroallergen-treated mice and its expression correlates with the asthma severity. In vivo blockade of PD-L2 reduces the severity of allergen-driven airway hyperresponsiveness (AHR).  Blockage of PD-L2 induces the IL-12 production by DC; IL-12 may limit the severity of allergen-induced AHR antagonizing IL-13. In addition, blockade of PD-1 does not have effect on AHR; therefore, PD-L2 controls asthma severity in a PD-1 independent manner. PD-L2 binds to a second receptor, RGMb (repulsive guidance molecule b) which is expressed in lung interstitial macrophages and alveolar epithelial cells and is a co-receptor for bone morphogenetic proteins BMP2\/4. Blockade of the RGMb\/PD-L2 interaction significantly impaired the development of respiratory tolerance since the initial T cell expansion is inhibited.\u003cbr\u003e\n25tests\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Recombinant human PD-L2 (B7-DC, CD273)-Fc chimera\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with PE\/Cyanine7 under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)Green Laser (532 nm)\/Yellow-Green Laser (561 nm)\u003cbr\u003e\nRRID: AB_2941641 (BioLegend Cat. No. 947809) AB_2941641 (BioLegend Cat. No. 947810)\u003cbr\u003e\nStructure: Dimer\u003cbr\u003e\nDistribution: Immature dendritic cells, mature dendritic cells, subset of macrophages, placental endothelium, and medullary thymic epithelial cells\u003cbr\u003e\nFunction: Inhibits T cell mediated proliferation and cytokine production by CD4+ T cells. Regulate T cell responses. PD-L2 expression is up-regulated on antigen-presenting cells by IFN-γ.\u003cbr\u003e\nInteraction: Antigen-stimulated T and B cells, regulatory T cells, follicular T and B cells, dendritic cells, monocytes\u003cbr\u003e\nLigand\/Receptor: PD-1 (CD279), RGMb\u003cbr\u003e\nCell Type: Antigen-presenting cells, Dendritic cells, Epithelial cells, Macrophages, Plasma cells\u003cbr\u003e\nBiology Area: Apoptosis\/Tumor Suppressors\/Cell Death, Cell Proliferation and Viability, Immunology\u003cbr\u003e\nMolecular Family: CD Molecules, Immune Checkpoint Receptors, Soluble Receptors\u003cbr\u003e\nAntigen References: Latchman Y, et al. 2001. Nat. Immunol. 2:261. Brown JA, et al. 2003. J. Immunol. 170:1257. Youngnak P, et al. 2003. Biochem. Biophys. Res. Commun. 307:672. Singh AK, et al. 2011. Allergy. 66:155. Lewkowich IP, et al. 2013. Mucosal. Immunol. 6:728. Xiao Y, et al. 2014. J. Exp. Med. 211:943. Bardhan K, et al. 2016. Front Immunol. 7:550.\u003cbr\u003e\nGene ID: 80380\u003cbr\u003e\nUniProt: View information about CD273 on UniProt.org\u003cbr\u003e\nClone: A20050B\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: CD273, Programmed cell death 1 ligand 2 (PD-L2), B7 dendritic cell molecule (B7-DC), Butyrophilin B7-DC, PDCD1 Ligand 2\u003cbr\u003e\nIsotype: Rat IgG2b, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866069291177,"sku":"947809","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-947809","provider":"Iright","version":"1.0","type":"link"}