{"product_id":"biolegend-948701","title":"Biolegend, 948701, Purified anti-CHOP Antibody, 25μg","description":"\u003cp\u003eWhen unfolded proteins accumulate in the ER lumen, multiple unfolded protein response (UPR) pathways become activated, including PERK, ATF6, and IRE1α, all of which transcriptionally reprogram the flux of proteins through the ER. When these quality control mechanisms fail to restore ER homeostasis, sustained stress results in the induction of CHOP, a pro-apoptotic transcription factor that negatively regulates expression of BCL2 and increases expression of BIM and TNFS10RB. One mechanism cancer cells exploit to adapt to harsh conditions of the tumor microenvironment is through upregulation of UPR pathways. Thus, understanding the molecular mechanisms that govern the switch from adaptation to apoptosis is of great therapeutic interest.\u003cbr\u003e\n25μg\u003cbr\u003e\nVerified Reactivity: Human, Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Partial recombinant human CHOP protein\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/mL\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: WB - Quality testedICC, ICFC - Verified\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 2.5 - 1.0 µg\/mL. For immunocytochemistry, a concentration 5.0 μg\/mL is recommended. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: This clone failed to detect CHOP in human small intestine.For ICC testing, we do not recommend methanol only as a fixation-permeabilization step due to poor CHOP staining. We recommend 4% PFA fixation with either Triton X-100 or methanol permeabilization.\u003cbr\u003e\nProduct Citations: Bietar K, et al. 2024. RSC Adv. 8695:14. PubMed\u003cbr\u003e\nRRID: AB_2904462 (BioLegend Cat. No. 948701) AB_2904462 (BioLegend Cat. No. 948702)\u003cbr\u003e\nStructure: CHOP is a 169 amino acid protein with a predicted molecular weight of 19 kD.\u003cbr\u003e\nDistribution: Expressed in cells and tissue undergoing ER stress\/Nucleus\u003cbr\u003e\nFunction: Transcription Factor\u003cbr\u003e\nBiology Area: Apoptosis\/Tumor Suppressors\/Cell Death, Cell Biology, Protein Misfolding and Aggregation, Transcription Factors\u003cbr\u003e\nAntigen References: 1. Yamaguchi, et al. 2004. J. Biol. Chem. 279: 45495 2. Oyadomari, et al. 2004. Cell Death Differ. 11:381. 3. Puthalakath H, et al. 2007. Cell. 129: 1337.\u003cbr\u003e\nGene ID: 1649\u003cbr\u003e\nUniProt: View information about CHOP on UniProt.org\u003cbr\u003e\nClone: 9C8\/CHOP\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: DDIT3, GADD153\u003cbr\u003e\nIsotype: Mouse IgG2b, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862506328233,"sku":"948701","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-948701","provider":"Iright","version":"1.0","type":"link"}