{"product_id":"biolegend-949602","title":"Biolegend, 949602, Purified anti-mouse CD44 Antibody, 100μg","description":"\u003cp\u003eCD44 is a 80-95 kD glycoprotein also known as Hermes, Pgp1, H-CAM, or HUTCH. It is expressed on all leukocytes, endothelial cells, hepatocytes, and mesenchymal cells. As B and T cells become activated or progress to the memory stage, CD44 expression increases from low or mid levels to high levels. Thus, CD44 has been reported to be a valuable marker for memory cell subsets. High CD44 expression on Treg cells has been associated with potent suppressive function via high production of IL-10. CD44 is an adhesion molecule involved in leukocyte attachment to and rolling on endothelial cells, homing to peripheral lymphoid organs and to the sites of inflammation, and leukocyte aggregation.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Partial recombinant mouse CD44 protein\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/mL\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: FC - Quality tested ICC, IHC-F - Verified\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. For immunocytochemistry, a concentration range of 2.5 - 5.0 μg\/mL is recommended. For immunohistochemical staining on frozen tissue sections, a concentration range of 1.125 - 5.0 µg\/mL is suggested It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: Not recommended for western blotting. Not reactive for human CD44 (tested in WB and IHC-P).\u003cbr\u003e\nRRID: AB_2922650 (BioLegend Cat. No. 949601) AB_2922650 (BioLegend Cat. No. 949602)\u003cbr\u003e\nStructure: Variable splicing of CD44 gene generates many CD44 isoforms, 80-95 kD\u003cbr\u003e\nDistribution: All leukocytes, epithelial cells, endothelial cells, hepatocytes, mesenchymal cells\u003cbr\u003e\nFunction: Leukocyte attachment and rolling on endothelial cells, stromal cells and ECM\u003cbr\u003e\nLigand\/Receptor: Hyaluronan, MIP-1β, fibronectin, collagen\u003cbr\u003e\nCell Type: B cells, Endothelial cells, Epithelial cells, Leukocytes, Mesenchymal cells\u003cbr\u003e\nBiology Area: Immunology\u003cbr\u003e\nMolecular Family: Adhesion Molecules, CD Molecules\u003cbr\u003e\nAntigen References: Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. Haynes BF, et al. 1991. Cancer Cells. 3:347-50. Goldstein LA, et al. 1989. Cell. 56:1063-72. Mikecz K, et al. 1995. Nat Med. 1:558-63. Hegde V, et al. 2008. J Leukoc Biol. 84:134-42. Liu T, et al. 2009. Biol Direct. 4:40.\u003cbr\u003e\nGene ID: 960\u003cbr\u003e\nUniProt: View information about CD44 on UniProt.org\u003cbr\u003e\nClone: W19210B\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Hermes, Pgp-1, H-CAM, HUTCH-1, ECMR III, gp85, Ly-24\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866346967209,"sku":"949602","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-949602","provider":"Iright","version":"1.0","type":"link"}