{"product_id":"biolegend-949802","title":"Biolegend, 949802, Purified anti-Caspase-3 Antibody, 100μg","description":"\u003cp\u003eCaspase 3 is a member of the peptidase family C14. This cytoplasmic caspase functions as a heterotetramer and has two isoforms (one acts as a dominant negative inhibitor). The pro-form of this caspase is approximately 32 kD. Caspase 3 is involved in the caspase activation cascade responsible for apoptosis execution. Caspase 3 is inhibited by c-IAP1, c-IAP2, XIAP, survivin, and Livin and activated by Caspase 6, -8, and -10, granzyme B, Apaf-1 by cleavage into large p17 and small p12 subunits. Caspase 3 has been shown to cleave PARP, huntingtin, androgen receptor, atrophin-1, DNA- PKcs, D4-GDI, hnRNPs C1\/C2, U1snRNP, PKC delta, ATM, fodrin, gelsolin, ICAD, RIP, X-IAP, STAT1, topoisomerase I, vimentin, Rb, lamin B, mdm2, and β-catenin.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Partial recombinant human Caspase-3 protein\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/mL\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: WB - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg\/mL. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: This antibody recognizes endogenous human Caspase-3 proteins in both total and spliced forms from Jurkat cell lysates treated with or without staurosporine.\u003cbr\u003e\nRRID: AB_2904464 (BioLegend Cat. No. 949801) AB_2904464 (BioLegend Cat. No. 949802)\u003cbr\u003e\nStructure: Caspase 3 is a 277 amino acid protein with a molecular mass of 32 kD.\u003cbr\u003e\nDistribution: Nucleus and cytosol\u003cbr\u003e\nFunction: Caspase 3 regulates programmed cell death (apoptosis)\u003cbr\u003e\nInteraction: Active heterodimers between large p17 subunit and small p11 subunit of Casp7 and vice versa. Cleaves PARP, huntingtin, androgen receptor, atrophin-1, DNA- PKcs, D4-GDI, hnRNPs C1\/C2, U1snRNP, PKC delta, ATM, fodrin, gelsolin, ICAD, RIP, X-IAP, STAT1, topo\u003cbr\u003e\nCell Type: B cells, Endothelial cells, Fibroblasts, Monocytes, T cells\u003cbr\u003e\nBiology Area: Apoptosis\/Tumor Suppressors\/Cell Death, Cell Biology, Cell Death, Neuroscience\u003cbr\u003e\nAntigen References: Goldberg YP, et al. 1996. Nat Genet. 13:442-9. Fernandes-Alnemri T, et al. 1996. Proc Natl Acad Sci U S A. 93:7464-9. Slee EA, et al. 2001. J Biol Chem. 276:7320-6. Woo M, et al. 2003. Nat Immunol. 4:1016-22.\u003cbr\u003e\nGene ID: 836\u003cbr\u003e\nUniProt: View information about Caspase-3 on UniProt.org\u003cbr\u003e\nClone: W20054B\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Caspase 3 preproprotein, Casp3, cpp32, Apopain, Yama, PARP cleavage protease, SREBP cleavage activity 1\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866419351721,"sku":"949802","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-949802","provider":"Iright","version":"1.0","type":"link"}