{"product_id":"cobetter-3550yh-r2703","title":"Cobetter, 3550YH-R2703, 0.2μm PVDF Transfer Membranes for Western Blot","description":"\u003cp\u003eCheck this product with a package size of 0.2μm \/ 275*3750mm \/ 1 roll\/pk on Iright. Cobetter PVDF transfer membranes feature high sensitivity and low background in Western blotting. The 0.45μm is suitable for most Western blotting applications and 0.2μm for immunoblotting of low MW proteins \u0026lt;20kDa.\u003cbr\u003e\nSpecifications\u003c\/p\u003e\n\n\u003cp\u003eMembrane\u003cbr\u003e\nHydrophobic PVDF\u003cbr\u003e\nPore Size\u003c\/p\u003e\n\n\u003cp\u003e0.2μm\u003cbr\u003e\n0.45μm\u003c\/p\u003e\n\n\u003cp\u003eThickness\u003c\/p\u003e\n\n\u003cp\u003e130μm\u003cbr\u003e\n125μm\u003c\/p\u003e\n\n\u003cp\u003eProtein Binding Capacity (BAS)\u003c\/p\u003e\n\n\u003cp\u003e300 µg\/cm²\u003cbr\u003e\n135 µg\/cm²\u003c\/p\u003e\n\n\u003cp\u003eDimension\u003cbr\u003e\n275mm x 3.75m\u003cbr\u003e\n84mm*70mm\u003cbr\u003e\n150mm*150mm\u003cbr\u003e\n200mm*200mm\u003cbr\u003e\nMain Binding Mechanisms\u003cbr\u003e\nElectrostatic, Hydrophobic\u003cbr\u003e\nPre-activation\u003cbr\u003e\nRequires ethanol (methanol)\u003cbr\u003e\nTolerance\u003cbr\u003e\nResistant to most organic solvents, acids and weak bases\u003cbr\u003e\nCompatibility\u003cbr\u003e\nCompatible with commonly used transfer conditions and detection methods (such as staining, chemiluminescence, radiolabeling, etc.)\u003cbr\u003e\nStorage\u003cbr\u003e\nStore the membrane flat at ambient temperature away from chemical vapors. Some solvent vapors may dissolve parts of the membrane, thereby disrupting the pore structure.\u003cbr\u003e\nPackage\u003cbr\u003e\n1 roll\/pk or 50pcs\/pk\u003cbr\u003e\nFeatures\u003cbr\u003e\nSmooth and flat surface, won't curl or fracture when cut\u003cbr\u003e\nHigh mechanical strength, easy to be stripped and reprobed multiple times\u003cbr\u003e\nUniform pore size, high protein mobility, clear and neat bands\u003cbr\u003e\nHigh sensitivity and low background, guarantee the success rate of low abundance protein detection\u003cbr\u003e\nApplications\u003cbr\u003e\nProtein transfer\u003cbr\u003e\nBlot detection\u003cbr\u003e\nAdsorption analysis\u003cbr\u003e\nPVDF Transfer Membrane Performance\u003cbr\u003e\nPrecautions\u003cbr\u003e\nPrevent bubbles from entering into the filter paper, gel or membrane\u003cbr\u003e\nFor samples containing small peptides, the equilibrium time of the gel in the transfer buffer should be limited to 10 minutes\u003cbr\u003e\nWhen the target protein is less than or equal to 15 kDa, 0.2μm PVDF transfer membrane can be used to obtain the maximum retention.\u003cbr\u003e\nThe PVDF transfer membrane is only used for scientific research by professionals, and should not be used for clinical diagnosis or treatment, food or medicine.\u003cbr\u003e\nFor your safety and health, please wear lab clothes and disposable gloves.\u003cbr\u003e\nRelated Articles\u003cbr\u003e\nPVDF Transfer Membranes in Western Blot: Case Studies in SCI Journals\u003cbr\u003e\nPVDF vs Nitrocellulose Membrane in Western Blot – Which One to Choose?\u003cbr\u003e\nWhy PVDF Transfer Membranes Are Ideal for Western Blotting Applications Q: Does Cobetter PVDF transfer membrane have a front and back side? Does it require activation before use?\u003cbr\u003e\nCobetter PVDF transfer membranes have a smooth and uniform surface and do not require distinguishing between the front and back sides during use.\u003cbr\u003e\nBefore use, simply pre-wet the membrane with absolute ethanol or absolute methanol. After activation, the membrane changes from white and opaque to semi-transparent. A soaking time of 30–60 seconds is recommended.\u003cbr\u003e\nQ: Are Cobetter PVDF transfer membranes available in pre-cut sizes?\u003cbr\u003e\nCobetter transfer membranes are available both in roll format and as boxed, pre-cut sheets, to meet different experimental needs.\u003cbr\u003e\nRoll format: 26.5 cm × 3.75 m\u003cbr\u003e\nPre-cut sheets: 200 mm × 200 mm; 84 mm × 70 mm\u003cbr\u003e\nQ: How to choose the appropriate pore size of PVDF transfer membranes?\u003cbr\u003e\nSelect the appropriate transfer membrane based on the molecular weight of the protein:\u003cbr\u003e\nSmall proteins (\u0026lt;20 kDa): use a 0.2 µm PVDF transfer membrane\u003cbr\u003e\nLarge proteins (\u0026gt;20 kDa): use a 0.45 µm PVDF transfer membrane\u003cbr\u003e\nQ: What should be considered when performing Western blotting for a high–molecular-weight protein (~200 kDa)?\u003cbr\u003e\nUse a lower-percentage resolving gel, preferably around 7%.\u003cbr\u003e\nHandle the gel carefully during stripping\/removal to avoid damage.\u003cbr\u003e\nExtend the transfer time to ensure efficient protein transfer.\u003cbr\u003e\nInclude a molecular weight marker, otherwise nonspecific bands may be difficult to interpret.\u003cbr\u003e\nQ: How should transfer conditions be set for Western blotting?\u003cbr\u003e\nFor proteins in the 15–100 kDa range, a typical condition is 300 mA constant current for 45 minutes.\u003cbr\u003e\nFor proteins \u0026gt;100 kDa, lower current (around 220 mA) is generally recommended with a longer transfer time.\u003cbr\u003e\nBased on experience: ZO-1 (250 kDa) can be successfully transferred in 2.5 hours. 150 kDa proteins can be transferred in 1.5 hours.\u003cbr\u003e\nFor example: If the largest target protein is 200 kDa, the transfer condition can be estimated as: 220 mA × (200 \/ 150) ≈ 134 minutes, plus an additional ~15–20 minutes, resulting in a total transfer time of approximately 150 minutes.\u003cbr\u003e\nQ: How can the background on the PVDF western blot membrane be reduced?\u003cbr\u003e\n1. Ensure proper blocking. PVDF membranes require more stringent blocking steps. This can be achieved by increasing the concentration of the blocking agent 2-5 fold, increasing the blocking time, nd performing the procedure at 37 degrees C.\u003cbr\u003e\n2. Optimizing the dilution ratios of the primary and secondary antibodies can reduce nonspecific background.\u003cbr\u003e\n3. Perform thorough washing, and keep the membrane continuously wet and free of contamination during all steps.\u003cbr\u003e\nQ: What are the advantages of Cobetter PVDF transfer membranes?\u003cbr\u003e\n1. Smooth and flat surface, resistant to curling.\u003cbr\u003e\n2. High mechanical strength, allowing easy stripping and repeated use.\u003cbr\u003e\n3. Uniform and consistent pore size, enabling high protein transfer efficiency and sharp, well-defined bands.\u003cbr\u003e\n4. Higher sensitivity, improving the detection success rate of low-abundance proteins\u003cbr\u003e\nQ: How should cellulose filter paper be selected for western blot?\u003cbr\u003e\nThe filter paper size should be slightly larger than the gel and the PVDF transfer membrane.\u003cbr\u003e\nCobetter pre-cut cellulose filter papers are designed to match the PVDF transfer membranes, offering convenient handling and an optimal fit.\u003c\/p\u003e","brand":"Cobetter","offers":[{"title":"Default Title","offer_id":46778379272361,"sku":"3550YH-R2703","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cobetter-3550yh-r2703","provider":"Iright","version":"1.0","type":"link"}