{"product_id":"cobetter-hfemi07501030p","title":"Cobetter, HFEMI07501030P, Biophsep MiniLab Hollow Fiber Filter mPES Membrane, Ultrafiltration, 1\/pk","description":"\u003cp\u003eCheck this product with a package size of 1.0mm \/ 30cm - EFA: 94 cm² \/ 750 kDa on Iright. By offering various lengths, membrane filtration areas, and module diameters, Cobetter hollow fiber modules can facilitate a smooth and predictable scale-up from laboratory to pilot-scale and eventually manufacturing-scale. Available from Mini, Minilab, Lab, Pilot, Pilot+, Process and Max.\u003cbr\u003e\nCobetter hollow fiber modules are made of modified polyethersulfone membranes(m-PES), which have high flow flux and low protein binding. They are easy to install, clean and sanitize, and are widely used in cell harvest, clarification, perfusion, diafiltration and concentration in upstream and downstream bioprocessing.\u003cbr\u003e\nCompare with TFF Cassette’s tortuous and complicated flow path, hollow fiber filtration has a linear flow path which creates a smooth flow for more gentle separation.\u003cbr\u003e\nSpecifications\u003c\/p\u003e\n\n\u003cp\u003eMaterials\u003cbr\u003e\nFeed\/Retentate connectors\u003cbr\u003e\nFemale Luer Lock\u003cbr\u003e\nPermeate connector\u003cbr\u003e\nFemale Luer lock\u003cbr\u003e\nMembrane material\u003cbr\u003e\nmPES\u003cbr\u003e\nHousing\u003cbr\u003e\nPolysulfone\u003cbr\u003e\nEnd Cap\u003cbr\u003e\nWhite-Polysulfone\u003cbr\u003e\nSeals (internal)\u003cbr\u003e\nEpoxy Resin\u003cbr\u003e\nPhysical Properties\u003cbr\u003e\nFiber ID\u003c\/p\u003e\n\n\u003cp\u003e0.5mm\u003cbr\u003e\n1.0mm\u003c\/p\u003e\n\n\u003cp\u003eNo. of Fibers\u003c\/p\u003e\n\n\u003cp\u003e25\u003cbr\u003e\n10\u003c\/p\u003e\n\n\u003cp\u003eEffective Length\u003c\/p\u003e\n\n\u003cp\u003e30cm60cm\u003cbr\u003e\n30cm60cm\u003c\/p\u003e\n\n\u003cp\u003eOD*L (mm*mm)\u003cbr\u003e\n30cm: 10*34660cm: 10*646\u003cbr\u003e\nEffective Area\u003c\/p\u003e\n\n\u003cp\u003e30cm: 118cm²60cm: 236cm²\u003cbr\u003e\n30cm: 94cm²60cm: 118cm²\u003c\/p\u003e\n\n\u003cp\u003eProcess Volume\u003c\/p\u003e\n\n\u003cp\u003e30cm: \u0026lt;1L60cm: \u0026lt;2L\u003cbr\u003e\n30cm: \u0026lt;1L60cm: \u0026lt;2L\u003c\/p\u003e\n\n\u003cp\u003eFlow Rate@2000s-1 (mL\/min)\u003c\/p\u003e\n\n\u003cp\u003e37\u003cbr\u003e\n118\u003c\/p\u003e\n\n\u003cp\u003eFlow Rate@6000s-1 (mL\/min)\u003c\/p\u003e\n\n\u003cp\u003e110\u003cbr\u003e\n353\u003c\/p\u003e\n\n\u003cp\u003eSterility\u003cbr\u003e\nDelivery Condition\u003cbr\u003e\nNonsterile\u003cbr\u003e\nPackage\u003cbr\u003e\nPack Size\u003cbr\u003e\n1 pc\/pk\u003cbr\u003e\nPackaging\u003cbr\u003e\nIndividually packed in PE bag\u003c\/p\u003e\n\n\u003cp\u003eFeatures\u003cbr\u003e\nHigh flux rates, high filtration capacity\u003cbr\u003e\nModified hydrophilic PES hollow fiber membrane provides low protein binding, less membrane fouling and easy cleaning\u003cbr\u003e\nIntegrated device without additional assembly or device holder, quick installation and operation\u003cbr\u003e\nRe-washed with 0.5M NaOH solution\u003cbr\u003e\nSimple and reliable linear scale-up\u003cbr\u003e\nApplications\u003cbr\u003e\nPurification, concentration and diafiltration of vaccine\u003cbr\u003e\nPurification, concentration and diafiltration of viral vector\u003cbr\u003e\nClarification of cells and bacterial in fermentation broth\u003cbr\u003e\nRecycling and washing of cells and bacteria\u003cbr\u003e\nConcentration and diafiltration of protein\u003cbr\u003e\nChoose Fiber ID\u003cbr\u003e\nModule with fiber of 0.5mm ID is widely used in most application scenarios to improve mass transfer efficiency.\u003cbr\u003e\nModule with fiber of 1.0mm ID is ideal for products with high cell density or high solid content or high viscosity.\u003cbr\u003e\nOnly module with fiber of 1.0mm ID is autoclavable, as steam cannot enter the fiber of 0.5mm ID effectively.\u003cbr\u003e\nShare Rate\u003cbr\u003e\nThe circulating flow rate of the hollow fiber depends on the product tolerance to the shear rate, in generally it will be set at 2000\/s to 10000\/s, which is much smaller than the shear rate generated by the turbulent flow on the surface of the cassette screen.\u003cbr\u003e\nFor general materials, we usually choose a shear rate of 4000\/s to 6000\/s;\u003cbr\u003e\nIf the product is sensitive to shear force (such as lentivirus, coronavirus or macromolecular protein expressed by animal cells, large plasmids and LNP, etc.), the shear rate needs to be reduced to 2000\/s;\u003cbr\u003e\nIf the product is with good resistance to shear force (such as small molecular proteins expressed by bacteria, etc.) shear rate could be increased to 8000\/s to 10000\/s.\u003cbr\u003e\nHow to choose hollow fiber MWCO?\u003cbr\u003e\nSufficient separation efficiency and the risks of fouling should both be taken into consideration when choosing MWCO.\u003cbr\u003e\nTo minimize the risk of fouling that inevitably occurs during membrane processing, it is advisable to choose membranes with relatively smaller pore sizes, as long as the separation efficiency and flux are maintained. This helps reduce the intrusion of impurity particles into the membrane pores, thus extending the membrane's service life.\u003cbr\u003e\nCommon processing scenarios are as follows:\u003cbr\u003e\nHeat source removal of small molecule in Chinese medicines: 5kD, 10kD\u003cbr\u003e\nUltrafiltration and concentration of mABs, buffer exchange: 30kD, 50kD\u003cbr\u003e\nConcentration, purification and removal of virus: 100kD, 300kD, 500kD, 750kD\u003cbr\u003e\nClarification of recombinant protein, antibody: 500kD, 750kD\u003cbr\u003e\nConcentration for bacteria: 500kD, 750kD\u003cbr\u003e\nClarification of lysate: 0.2μm, 0.45μm, 0.65μm\u003cbr\u003e\nEffective Length and Scale-Up\u003cbr\u003e\nThe process scale-up feature of hollow fibers is that: direct process scale-up can be carried out as long as the effective length is kept the same.\u003cbr\u003e\nOn the opposite, due to the significant pressure drop difference between inlet and outlet existed in different lengths, the internal pressure and flow velocity distribution of the flow channel also change correspondingly, so linear scale-up can not be performed on devices with different length. When processing with more fouling and high viscosity product, it is preferred to choose module with shorter length.\u003cbr\u003e\nHow to use a hollow fiber membrane filter?\u003cbr\u003e\nModule Preparation\u003cbr\u003e\nFor lab and pilot applications, you will need a pump, a feed tank, a permeate collection tank, pressure gauges and valves and suitable tubing and connectors.Cobetter hollow fiber modules made with hydrophilic modified PES membrane contain glycerin to preserve the pore structure. Glycerine is water soluble and easy to rinse out.\u003cbr\u003e\nRinse out glycerine with DI water with an inlet pressure of 2-5 psi, the flushing volume should be greater than 20L\/m².\u003cbr\u003e\nDrain module and perform module integrity test.\u003cbr\u003e\n Note: If sanitization is necessary, use 0.1N NaOH solution as a disinfectant.\u003cbr\u003e\nModule Integrity Test\u003cbr\u003e\nAll hollow fiber modules are integrity tested at our site. However, Cobetter highly recommends an integrity test before use. Cobetter recommends two test methods, one of which may be applied according to actual operating conditions.  \u003cbr\u003e\nDiffusion Flow Method\u003cbr\u003e\nPressure Retaining Method\u003cbr\u003e\nProcessing Buffer Equilibrium\u003cbr\u003e\nThe purpose of flushing hollow fiber modules with buffer is to ensure that the pH and salt ion conditions of the system are as similar as possible to the initial state of the sample, so as to reduce pollution and product loss.\u003cbr\u003e\nCleaning after Use\u003cbr\u003e\nThe hollow fiber modules must be cleaned before storage and reused by flushing with buffer or DI water, and then washed with appropriate sanitizing agent. 0.5M NaOH solution may be used to circulate for 30 min at 20-40°C.\u003cbr\u003e\nHollow Fiber Storage\u003cbr\u003e\nThe hollow fiber modules may be stored in 0.1N NaOH solution. For long-term storage between 1-12 months, it is recommended to be stored under refrigeration at 4. Q: What are the advantages of hollow fiber compared to cassette (flat-sheet) modules?\u003cbr\u003e\n1. More open flow channels and relatively lower shear.\u003cbr\u003e\n2. High packing density and large surface area per module enable a compact system footprint with minimal hold-up volume.\u003cbr\u003e\n3. Robust handling of challenging feed streams, including high solids content or viscosity.\u003cbr\u003e\n4. Suited for large-volume, high-flow continuous processing in drug manufacturing, such as for insulin and animal vaccines.\u003cbr\u003e\nQ: What are the applications of hollow fiber technology?\u003cbr\u003e\nConcentration and buffer exchange of viral vaccines.\u003cbr\u003e\nConcentration and diafiltration of Cell and Gene Therapy (CGT) products.\u003cbr\u003e\nPurification, concentration, and buffer exchange of mRNA products.\u003cbr\u003e\nBiomass harvesting from bacterial fermentation, and clarification\/removal of cell debris from cell culture lysates.\u003cbr\u003e\nCell retention in perfusion culture (ATF system).\u003cbr\u003e\nQ: What materials and cut-offs are available for Cobetter ultrafiltration hollow fiber?\u003cbr\u003e\nMaterial: m-PES.\u003cbr\u003e\nUltrafiltration HF are available cut-offs ranging from 3–750 kDa.\u003cbr\u003e\nMicrofiltration HF are available pore sizes ranging from 0.1–0.65 µm.\u003cbr\u003e\nQ: Can Cobetter‘s hollow fiber modules be sterilized?\u003cbr\u003e\nSpecific series are compatible with sterilization procedures, for example, 50 kDa, 750 kDa, and 0.2 μm modules.\u003c\/p\u003e","brand":"Cobetter","offers":[{"title":"Default Title","offer_id":46778363117737,"sku":"HFEMI07501030P","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cobetter-hfemi07501030p","provider":"Iright","version":"1.0","type":"link"}