{"product_id":"cobetter-hfepr03000560sp","title":"Cobetter, HFEPR03000560SP, Biophsep Process Hollow Fiber Filter Set with Bag Ultrafiltration, Gamma Sterilized 1\/pk","description":"\u003cp\u003eCheck this product with a package size of 0.5mm \/ 60cm - EFA: 4.90 m² \/ 300 kDa on Iright. Designed for single use, eliminating the need for post-use ClP cleaning, regeneration, and cleaning validation. This significantly saves operational time and avoids the risk of cross-contamination.\u003cbr\u003e\nPre-treated to reduce TOC and conductivity levels, effectively minimizing endotoxin and bioburden. No need for pre-use alkaline washing to remove pyrogens, enabling rapid experimental setup. Each filter is individually packaged and gamma-irradiated before shipment to ensure sterility.\u003cbr\u003e\nSpecifications\u003c\/p\u003e\n\n\u003cp\u003eMaterials\u003cbr\u003e\nFeed\/Retentate connectors\u003cbr\u003e\n1.5\" TC (compatible with 1-1\/2\" TC fitting)\u003cbr\u003e\nPermeate connector\u003cbr\u003e\n1.0\" TC (compatible with 1-1\/2\" TC fitting)\u003cbr\u003e\nMembrane material\u003cbr\u003e\nmPES\u003cbr\u003e\nHousing\u003cbr\u003e\nPolysulfone\u003cbr\u003e\nEnd Cap\u003cbr\u003e\nWhite-Polysulfone\u003cbr\u003e\nSeals (internal)\u003cbr\u003e\nEpoxy Resin\u003cbr\u003e\nPhysical Properties\u003cbr\u003e\nFiber ID\u003c\/p\u003e\n\n\u003cp\u003e0.5mm\u003cbr\u003e\n1.0mm\u003c\/p\u003e\n\n\u003cp\u003eNo. of Fibers\u003c\/p\u003e\n\n\u003cp\u003e5,200\u003cbr\u003e\n2,200\u003c\/p\u003e\n\n\u003cp\u003eEffective Length\u003c\/p\u003e\n\n\u003cp\u003e30cm60cm110cm\u003cbr\u003e\n30cm60cm110cm\u003c\/p\u003e\n\n\u003cp\u003eOD*L (mm*mm)\u003cbr\u003e\n30cm: 89*47760cm: 89*777110cm: 89*1,227\u003cbr\u003e\nEffective Area\u003c\/p\u003e\n\n\u003cp\u003e30cm: 2.45m²60cm: 4.90m²110cm: 8.57m²\u003cbr\u003e\n30cm: 2.08m²60cm: 4.16m²110cm: 7.25m²\u003c\/p\u003e\n\n\u003cp\u003eProcess Volume\u003c\/p\u003e\n\n\u003cp\u003e30cm: \u0026lt;200L60cm: \u0026lt;500L110cm: \u0026lt;800L\u003cbr\u003e\n30cm: \u0026lt;200L60cm: \u0026lt;500L110cm: \u0026lt;800L\u003c\/p\u003e\n\n\u003cp\u003eFlow Rate@2000s-1 (mL\/min)\u003c\/p\u003e\n\n\u003cp\u003e7,647\u003cbr\u003e\n25,880\u003c\/p\u003e\n\n\u003cp\u003eFlow Rate@6000s-1 (mL\/min)\u003c\/p\u003e\n\n\u003cp\u003e22,941\u003cbr\u003e\n77,650\u003c\/p\u003e\n\n\u003cp\u003eSterility\u003cbr\u003e\nDelivery Condition\u003cbr\u003e\nSterile (Single use)\u003cbr\u003e\nSterilization Method\u003cbr\u003e\nGamma Irradiation at 25-45 kGy\u003cbr\u003e\nPackage\u003cbr\u003e\nPack Size\u003cbr\u003e\n1 pc\/pk\u003cbr\u003e\nPackaging\u003cbr\u003e\nIndividually packed in PE bag\u003c\/p\u003e\n\n\u003cp\u003eFeatures\u003cbr\u003e\nPre-sterilized with gamma irradiation at an intensity of 25-45 kGy\u003cbr\u003e\nPre-treated to reduce conductivity and TOC residue, minimizing wetting and shortening installation time\u003cbr\u003e\nSingle-use design eliminates the need for post-use ClP cleaning\u003cbr\u003e\nApplications\u003cbr\u003e\nYeast, oncolytic bacteria, and other cell harvesting\/purification\u003cbr\u003e\nContinuous cell perfusion culture\u003cbr\u003e\nAseptic concentration of plasmids and mRNA in CGT applications\u003cbr\u003e\nAseptic purification of recombinant proteins\u003cbr\u003e\nHollow Fiber Filter Configurations Compared\u003cbr\u003e\nSterilization Compatibility Comparison\u003cbr\u003e\nChoose Fiber ID\u003cbr\u003e\nModule with fiber of 0.5mm ID is widely used in most application scenarios to improve mass transfer efficiency.\u003cbr\u003e\nModule with fiber of 1.0mm ID is ideal for products with high cell density or high solid content or high viscosity.\u003cbr\u003e\nOnly module with fiber of 1.0mm ID is autoclavable, as steam cannot enter the fiber of 0.5mm ID effectively.\u003cbr\u003e\nShare Rate\u003cbr\u003e\nThe circulating flow rate of the hollow fiber depends on the product tolerance to the shear rate, in generally it will be set at 2000\/s to 10000\/s, which is much smaller than the shear rate generated by the turbulent flow on the surface of the cassette screen.\u003cbr\u003e\nFor general materials, we usually choose a shear rate of 4000\/s to 6000\/s;\u003cbr\u003e\nIf the product is sensitive to shear force (such as lentivirus, coronavirus or macromolecular protein expressed by animal cells, large plasmids and LNP, etc.), the shear rate needs to be reduced to 2000\/s;\u003cbr\u003e\nIf the product is with good resistance to shear force (such as small molecular proteins expressed by bacteria, etc.) shear rate could be increased to 8000\/s to 10000\/s.\u003cbr\u003e\nHow to choose hollow fiber MWCO?\u003cbr\u003e\nSufficient separation efficiency and the risks of fouling should both be taken into consideration when choosing MWCO.\u003cbr\u003e\nTo minimize the risk of fouling that inevitably occurs during membrane processing, it is advisable to choose membranes with relatively smaller pore sizes, as long as the separation efficiency and flux are maintained. This helps reduce the intrusion of impurity particles into the membrane pores, thus extending the membrane's service life.\u003cbr\u003e\nCommon processing scenarios are as follows:\u003cbr\u003e\nHeat source removal of small molecule in Chinese medicines: 5kD, 10kD\u003cbr\u003e\nUltrafiltration and concentration of mABs, buffer exchange: 30kD, 50kD\u003cbr\u003e\nConcentration, purification and removal of virus: 100kD, 300kD, 500kD, 750kD\u003cbr\u003e\nClarification of recombinant protein, antibody: 500kD, 750kD\u003cbr\u003e\nConcentration for bacteria: 500kD, 750kD\u003cbr\u003e\nClarification of lysate: 0.2μm, 0.45μm, 0.65μm\u003cbr\u003e\nEffective Length and Scale-Up\u003cbr\u003e\nThe process scale-up feature of hollow fibers is that: direct process scale-up can be carried out as long as the effective length is kept the same.\u003cbr\u003e\nOn the opposite, due to the significant pressure drop difference between inlet and outlet existed in different lengths, the internal pressure and flow velocity distribution of the flow channel also change correspondingly, so linear scale-up can not be performed on devices with different length. When processing with more fouling and high viscosity product, it is preferred to choose module with shorter length. Q: What are the advantages of hollow fiber compared to cassette (flat-sheet) modules?\u003cbr\u003e\n1. More open flow channels and relatively lower shear.\u003cbr\u003e\n2. High packing density and large surface area per module enable a compact system footprint with minimal hold-up volume.\u003cbr\u003e\n3. Robust handling of challenging feed streams, including high solids content or viscosity.\u003cbr\u003e\n4. Suited for large-volume, high-flow continuous processing in drug manufacturing, such as for insulin and animal vaccines.\u003cbr\u003e\nQ: What are the applications of hollow fiber technology?\u003cbr\u003e\nConcentration and buffer exchange of viral vaccines.\u003cbr\u003e\nConcentration and diafiltration of Cell and Gene Therapy (CGT) products.\u003cbr\u003e\nPurification, concentration, and buffer exchange of mRNA products.\u003cbr\u003e\nBiomass harvesting from bacterial fermentation, and clarification\/removal of cell debris from cell culture lysates.\u003cbr\u003e\nCell retention in perfusion culture (ATF system).\u003cbr\u003e\nQ: What materials and cut-offs are available for Cobetter ultrafiltration hollow fiber?\u003cbr\u003e\nMaterial: m-PES.\u003cbr\u003e\nUltrafiltration HF are available cut-offs ranging from 3–750 kDa.\u003cbr\u003e\nMicrofiltration HF are available pore sizes ranging from 0.1–0.65 µm.\u003cbr\u003e\nQ: Can Cobetter‘s hollow fiber modules be sterilized?\u003cbr\u003e\nSpecific series are compatible with sterilization procedures, for example, 50 kDa, 750 kDa, and 0.2 μm modules.\u003c\/p\u003e","brand":"Cobetter","offers":[{"title":"Default Title","offer_id":46778489897129,"sku":"HFEPR03000560SP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cobetter-hfepr03000560sp","provider":"Iright","version":"1.0","type":"link"}