{"product_id":"cobetter-ufelaa100l002p","title":"Cobetter, UFELAA100L002P, Consieve Tangential Flow Filtration Cassette PES Ultrafiltration Membrane Lab, 1\/pk","description":"\u003cp\u003eCheck this product with a package size of 0.02 m² \/ 100 kDa (high flux) \/ Tight Screen on Iright. Consieve™ UET tangential flow filtration cassette is made of asymmetric mPES (modified Polyethersulfone) membrane, which provides high flow rate, low protein bindings and wide pH compatibility. The cassette is easy to clean, install and has a high retention efficiency, with low working volume and high efficiency which ensures product yields.\u003cbr\u003e\nConsieve™ UET ultrafiltration TFF cassettes are available in a wide range of molecular weight cut-offs from 1 kDa to 1000 kDa. It can be easily scale-up from Lab, Pilot to Process scale with a wide volume range from 0.5L to 20,000L. They are widely used in separation, concentration and diafiltration in downstream bioprocessing. \u003cbr\u003e\nThe cassettes should be used with Cobetter Stainless Steel TFF Cassette Holder:\u003cbr\u003e\nEFA 0.01m² or 0.02m² cassettes use with Ultrafiltration Cassette Holders (0.01m²)\u003cbr\u003e\nEFA 0.02m² or 0.11m² cassettes use with Ultrafiltration Cassette Holders (0.11m²)\u003cbr\u003e\nEFA 0.5m² or 2.5m² cassettes use with Ultrafiltration Cassette Holders (0.5m²)\u003cbr\u003e\nSpecifications\u003c\/p\u003e\n\n\u003cp\u003eMaterials\u003cbr\u003e\nMembrane material\u003cbr\u003e\nmPES\u003cbr\u003e\nScreen\u003cbr\u003e\nPP\u003cbr\u003e\nSeals(internal)\u003cbr\u003e\nSilicone\u003cbr\u003e\nEncapsulant\u003cbr\u003e\nWhite polyurethane Silicone\u003cbr\u003e\nGaskets\u003cbr\u003e\nPlatinum cured silicone\u003cbr\u003e\nPhysical Properties\u003cbr\u003e\nMWCO\u003cbr\u003e\n1\/3\/5\/8\/10\/30\/50\/100\/300\/500\/1000 kDa\u003cbr\u003e\nPressure Drop\u003cbr\u003e\n0.3-0.6 bar (Circulation Flow Rate 6L\/min\/m²)\u003cbr\u003e\nEffective Filtration Area\u003c\/p\u003e\n\n\u003cp\u003e0.01 m² (0.11 ft²)\u003cbr\u003e\n0.11 m² (1.2 ft²)\u003c\/p\u003e\n\n\u003cp\u003eThroughput\u003c\/p\u003e\n\n\u003cp\u003e200-2000mL\u003cbr\u003e\n5-50L\u003c\/p\u003e\n\n\u003cp\u003eDead Volume\u003c\/p\u003e\n\n\u003cp\u003e2mL\u003cbr\u003e\n12mL\u003c\/p\u003e\n\n\u003cp\u003eDiffusion Flow @0.1Mpa\u003c\/p\u003e\n\n\u003cp\u003e≤1.1 mL\/min\u003cbr\u003e\n≤12 mL\/min\u003c\/p\u003e\n\n\u003cp\u003eCorssflow Rate @0.2Mpa\u003c\/p\u003e\n\n\u003cp\u003e77-154 mL\/min\u003cbr\u003e\n  0.84-1.68 L\/min\u003c\/p\u003e\n\n\u003cp\u003eOperating Conditions\u003cbr\u003e\nMax. Operating Temperature\u003cbr\u003e\n50°C\u003cbr\u003e\nMax. Operating Pressure\u003cbr\u003e\n4.0 bar\u003cbr\u003e\npH Range\u003cbr\u003e\n1.0-14.0\u003cbr\u003e\nSterility\u003cbr\u003e\nDelivery Condition\u003cbr\u003e\nNonsterile\u003cbr\u003e\nPackage\u003cbr\u003e\nPack Size\u003cbr\u003e\n1 pcs\u003cbr\u003e\nPackaging\u003cbr\u003e\nIndividually packed in PE bag\u003c\/p\u003e\n\n\u003cp\u003eScreen Difference\u003c\/p\u003e\n\n\u003cp\u003eScreen Type\u003cbr\u003e\nFlow Resistance\u003cbr\u003e\nFlow Rate (LMM@dp 15psi Water)\u003cbr\u003e\nApplications\u003cbr\u003e\nTight Screen\u003cbr\u003e\nHigh\u003cbr\u003e\n5~6\u003cbr\u003e\nGenerates the strongest turbulence on the membrane surface. Tight Screen can achieve relatively high tangential flow velocity at a relatively low inlet flow rate, but has a high pressure drop (dp) and is easily clogged by particles. Tight screen cassettes are suitable for samples without particles, low viscosity, and low concentration.\u003cbr\u003e\nStandard Coarse Screen\u003cbr\u003e\nMedium\u003cbr\u003e\n8~11\u003cbr\u003e\nGenerates moderate turbulence on the membrane surface, reducing clogging. Standard Coarse Screen requires a higher inlet flow rate to match the appropriate tangential flow velocity. The pressure drop (dp) is significantly lower compared to Tight Screens. Standard coarse screen cassettes are suitable for clarified samples with medium to high viscosity.\u003cbr\u003e\nSuspended Screen\u003cbr\u003e\nLow\u003cbr\u003e\n≥40\u003cbr\u003e\nGenerates the weakest turbulence and low shear force on the surface, but still significantly reduces clogging. Theoretically, Suspended Screen requires a very high inlet flow rate. Due to its open screen structure, it can accommodate more particles (cells, cell debris) without clogging. Suspended screen cassettes are suitable for unclarified samples with high particle content or high viscosity, and samples that are sensitive to shear force.\u003cbr\u003e\nFeatures\u003cbr\u003e\nHigh flow rate\u003cbr\u003e\nLow protein binding\u003cbr\u003e\nHigh product yield\u003cbr\u003e\nNo glue used, resulting in low extractables\u003cbr\u003e\npH range: 1-14\u003cbr\u003e\nApplications\u003cbr\u003e\nConcentration and desalting of proteins, peptides or nucleic acids\u003cbr\u003e\nBuffer exchange\u003cbr\u003e\nFractionation and purification\u003cbr\u003e\nSample preparation before chromatography\u003cbr\u003e\nRemove pyrogens from water, buffers, media solutions Q: What is tangential flow filtration (TFF)?\u003cbr\u003e\nTangential flow filtration (TFF) is a method in which the feed flows along the membrane surface, generating tangential shear to reduce membrane fouling and clogging. TFF is commonly applied as ultrafiltration (1–1000 kDa) or microfiltration (0.1–0.65 µm).\u003cbr\u003e\nQ: How to choose the appropriate ultrafiltration membranes?\u003cbr\u003e\nFor most ultrafiltration and dialysis applications, both PES and RC membranes are suitable choices.\u003cbr\u003e\nPES membranes can meet a wide range of application needs for most protein and small-molecule products, such as antibodies, APIs, and vaccines.\u003cbr\u003e\nRC membranes should be considered when preparing high-concentration formulations, applications containing organic solvents (e.g., ADC products), or concentration\/exchange of linear molecules including oligonucleotides and peptides.\u003cbr\u003e\nFor new target molecules, it is recommended to test both membrane types during standard sample handling and process optimization.\u003cbr\u003e\nQ: How to select the appropriate cut-off for ultrafiltration cassettes?\u003cbr\u003e\nChoose the membrane cut-off based on the size or molecular weight of the substance to be separated or retained:\u003cbr\u003e\nAntibody proteins: 30 kDa or 50 kDa\u003cbr\u003e\nViral or vaccine products: 100–1000 kDa\u003cbr\u003e\nFor API products, an appropriate molecular weight cut-off (MWCO) can be selected from the range of 1 kDa, 2 kDa, 3 kDa, 5 kDa, 8 kDa, and 10 kDa according to the process objectives.\u003cbr\u003e\nIf the molecular weight of the target substance is unknown, preliminary testing can be performed using ultrafiltration centrifugal devices with different cut-offs to identify the most suitable molecular weight retention.\u003cbr\u003e\nQ: What materials and cut-offs are available for Cobetter cassettes?\u003cbr\u003e\nUltrafiltration cassettes are available in PES and RC membranes, with cut-offs ranging from 1–1000 kDa.\u003cbr\u003e\nMicrofiltration cassettes are available in PES and PVDF membranes, with pore sizes ranging from 0.1–0.65 µm.\u003cbr\u003e\nQ: Can ultrafiltration cassettes be reused?\u003cbr\u003e\nYes. Ultrafiltration cassettes can be reused by selecting appropriate cleaning methods. However, due to changes such as declining membrane flux and increased permeation, the cassettes should be replaced periodically as needed.\u003cbr\u003e\nQ: How should ultrafiltration cassettes be cleaned?\u003cbr\u003e\nThey can be cleaned by circulating 0.1-0.5 M sodium hydroxide (NaOH) solution. If cleaning effectiveness is insufficient, adjustments can be made by either of the following methods:\u003cbr\u003e\nIncreasing the cleaning solution temperature (\u0026lt;45 °C)\u003cbr\u003e\nAdding sodium hypochlorite (NaClO, 500 ppm)\u003cbr\u003e\nExtending the cleaning time\u003cbr\u003e\nFor difficult-to-clean feed components, alternative cleaning solutions such as acidic solutions, alcohols, or surfactants may also be used.\u003cbr\u003e\nQ: Are Cobetter ultrafiltration membrane cassettes compatible with hardware from other companies?\u003cbr\u003e\nYes. They can be installed in holders from Merck, Pall, and similar manufacturers. But it is necessary to ensure proper torque and perform integrity testing.\u003cbr\u003e\nFor Sartorius holders, a specific adapter plate is required for proper installation.\u003c\/p\u003e","brand":"Cobetter","offers":[{"title":"Default Title","offer_id":46778369802409,"sku":"UFELAA100L002P","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cobetter-ufelaa100l002p","provider":"Iright","version":"1.0","type":"link"}