{"product_id":"cobetter-ulrc0300150p-4","title":"Cobetter, ULRC0300150P-4, Briscale 15mL Ultrafiltration Centrifugal Filter with Regenerated Cellulose RC Membrane","description":"Check this product with a package size of 30 kDa \/ 7.4cm² \/ 4\/pk on Iright. Cobetter BriScale 15mL centrifugal filters are made of low-binding regenerated cellulose(RC) ultrafiltration membranes, which can achieve a high protein recovery rate \u0026gt;90%, using marker protein Lysozyme(14.4 kDa) at 0.25 mg\/mL, centrifuge 60 mins.\nBriScale centrifugal filter is ideal for the concentration of proteins and biological samples containing antigens, antibodies, enzymes, nucleic acids, or microorganisms. The vertical design and membrane surface area provide fast concentration time and rapid protein concentration for up to 80-fold concentration.\nThe centrifugal filter unit consists of a lid, a filter device and a centrifuge tube. Use a pipette to collect the concentrate from the filter device. The ultrafiltrate is collected in the centrifuge tube for further analysis.\nBriScale 15 RC Centrifugal Filters can handle samples up to 15 mL in rotors accepting 50 mL centrifuge tubes. Use in fixed angle 45°rotor(max starting volume of 12mL) or swing bucket rotor(max starting volume of 15mL).\nSpecifications\n\nDimensions\nEffective membrane area\n7.4cm²\nOverall Length x Tube diameter\n119.6 x 29 mm\nMaterials\nMembrane Material\nRegenerated Cellulose (RC)\nCentrifuge Tube\nPP\nUltrafiltration device\nPolystyrene (GPPS)\nLid\nHigh Density Polyethylene (HDPE)\nConcentrator Capacity\nFixed Angle Rotor(45°)\nSwing Bucket Rotor\n\nMaximum Sample Volume\n\n12 mL\n15 mL\n\nDead-stop volume\n\n60 μL\n100 μL\n\nFinal concentration volume\n150-300μL\nCentrifuge\nFixed Angle Rotor(45°)\nSwing Bucket Rotor\n\nMaximum G-force\n\n5000g\n4000g\n\nSterility\nDelivery Condition\nNonsterile\nSterilization Method\nNot Recommended\nPackage\nPack Size\n4pcs or 24pcs\nPackaging\nIndividually packed in PE bag\nFeatures\nRC membrane achieves a high recovery rate of\u0026gt;90%\nColor-coded cap with different MWCO, volume graduation for easy detection and user-friendly\nLow-binding regenerated cellulose(RC) ultrafiltration membranes\nBroad chemical compatibility pH 1-14\n80-fold concentration with vertical membranes\nTranslucent housing and volume marker make sample monitoring effortless\nApplications\nConcentration and purification of nucleic acids, proteins such as antigens, antibodies, enzymes, etc and other biomolecules\nDesalting, buffer exchange and dialyzing proteins and antibodies for sample preparation\nConcentration of dilute or pre-purified proteins from chromatography\nRemove primers, adapters or molecular tags from the reaction mixture\nPurification of macromolecules in tissue culture extracts or cell lysates, and protein removal prior to HPLC and mass spectrometry\nHow to choose Cobetter Centrifugal Filters?\nTo choose which type to use, determine the molecular weight (MW) of your macromolecule of interest, and select an ultrafiltration membrane with a molecular weight cut-off (MWCO) that is 2-3 times smaller than the MW of your macromolecule. Read more details about how to choose MWCO filters.\n\nFilter MWCO\nTarget MW\n3 kDa\n6 kDa \u0026lt; MW \u0026lt; 30 kDa\n10 kDa\n30 kDa \u0026lt; MW \u0026lt; 100 kDa\n30 kDa\n100 kDa \u0026lt; MW \u0026lt; 200 kDa\n50 kDa\n200 kDa \u0026lt; MW \u0026lt; 500 kDa\n100 kDa\n500 kDa \u0026lt; MW\n\nCentrifugal Filter Units Structure \u0026amp; Adapted Equipment\nCobetter ultrafiltration centrifugal filter units consist of a lid, a filter unit, and a centrifuge tube.\nIt requires a rotor or rotor adapter compatible with 15mL (for 4mL centrifugal filter) or 50mL (for 15mL centrifugal filter) conical bottom centrifuge tubes. Both the swinging bucket rotor and the fixed-angle rotor must meet this requirement. Learn more about how to use a centrifugal filter.\nProtein Recovery Rate\nThe RC membrane and smooth inner wall design have extremely low protein adsorption. Can achieve a recovery rate of more than 90%. Q: Which centrifuges are Cobetter centrifugal filters compatible with?\nBriscale UF-15 centrifugal filters are compatible with centrifuges that accept 50 mL conical centrifuge tubes.\nBriscale UF-4 centrifugal filters are compatible with centrifuges that accept 15 mL conical centrifuge tubes.\nBoth are suitable for horizontal rotors and fixed-angle rotors.\nQ: How to choose the appropriate centrifugal filters?\nWhen selecting a centrifugal filter, the main considerations are the molecular weight of the target molecule and the desired concentration volume. For selecting the membrane cut-off, a general guideline is to choose a cut-off no greater than 1\/3 of the target protein’s molecular weight.\nFor example, if the target protein has a molecular weight of 35 kDa, an ultrafiltration tube with a 10 kDa cut-off would be appropriate.\nQ: How should centrifugal filters be cleaned and stored?\nCleaning:\nSoak the tube in 0.1–0.5 N NaOH solution for 30–60 minutes. Discard the NaOH solution, then centrifuge with ultrapure or deionized water for 10 minutes, repeating 1–2 times.\nStorage:\nFill the tube with 0.05–0.1 N NaOH solution and store immersed.\nQ: Are Cobetter centrifugal filter single-use? Can they be reused?\nCobetter Briscale UF ultrafiltration centrifugal filters are single-use consumables.\nFor the same feed material, they can be reused 6–10 times with 30 min centrifugation per use without issues.\nFor different feed materials, it is recommended to use a new centrifugal filter.\nQ: Can Cobetter centrifugal filters be sterilized?\nCobetter Briscale UF ultrafiltration centrifugal filters are non-sterile and\ncannot\nbe autoclaved. They can be disinfected using 0.1–0.5 N NaOH or 70% ethanol.\nQ: What cause low recovery rate after ultrafiltration?\nThere are four possible reasons:\n1. Inappropriate ultrafiltration cut-off leading to product loss: Try using a smaller cut-off, generally following the 1\/3 rule. For example, for a 10 kDa target molecule, the preferred cut-off is 3 kDa, while also testing 10 kDa and 30 kDa tubes to compare recovery.\n2. Excessive concentration factor leading to incomplete recovery: Consider reducing centrifugation time and controlling the sample load.\n3. Product integrity failure: Compare two centrifugal filters using buffer or pure water. A damaged centrifugal filter typically shows faster flow and lower concentrated volume.\n4. Adsorption effects: If the initial sample concentration is below 0.1 mg\/mL, adsorption inside the tube may reduce recovery.\nQ: Why does protein precipitation occur during concentration?\nProtein precipitation may occur when the protein is concentrated too rapidly or to an excessively high concentration. Recommended optimization strategies:\n1. Reduce the loading volume or centrifugation time to lower the final protein concentration.\n2. Decrease the centrifugal force or centrifugation speed.\n3. Increase mixing steps, breaking a single long centrifugation into multiple shorter centrifugation cycles.","brand":"Cobetter","offers":[{"title":"Default Title","offer_id":46778334675113,"sku":"ULRC0300150P-4","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cobetter-ulrc0300150p-4","provider":"Iright","version":"1.0","type":"link"}