CST, 10076S, FADS1 (F1D2A) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying FADS1. Validated for Western Blotting,Simple Western™,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, FADS1 (F1D2A) Rabbit Monoclonal Antibody (CST #10076) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:50 - 1:250 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity FADS1 (F1D2A) Rabbit Monoclonal Antibody recognizes endogenous levels of total FADS1 protein. This antibody does not cross-react with FADS2 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Phe100 of human FADS1 protein. Background Fatty acid desaturase 1 (FADS1), also known as delta-5 desaturase, is essential for the biosynthesis of highly unsaturated fatty acids (HUFAs) from linoleic acid (LA) and α-linolenic acid (ALA), both of which are polyunsaturated fatty acids (PUFAs). In addition, FADS1 converts NADH to NAD . Studies show that FADS1 and fatty acid desaturase 2 (FADS2) thus enable NAD recycling for glycolysis to support cell viability when aerobic respiration is acutely suppressed (1,2). Furthermore, FADS1 and ELOVL5 expression is enhanced in mesenchymal-type gastric cancer cells, sensitizing the cancer cells to ferroptosis. On the contrary, FADS1 and ELOVL5 expression is inhibited in intestinal-type gastric cancer cells, leading to resistance to ferroptosis (3). Alternate Names Acyl-CoA (8-3)-desaturase; D5D; delta-5 desaturase; delta-5 fatty acid desaturase; Delta(5) desaturase; Delta(5) fatty acid desaturase; FADS1; FADS6; FADSD5; Fatty acid desaturase 1; FLJ38956; FLJ90273; linoleoyl-CoA desaturase (delta-6-desaturase)-like 1; LLCDL1; TU12 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 43 Source/Isotype: Rabbit IgG