{"product_id":"cst-10076t","title":"CST,  10076T, FADS1 (F1D2A) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying FADS1. Validated for Western Blotting,Simple Western™,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, FADS1 (F1D2A) Rabbit Monoclonal Antibody (CST #10076) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nSimple Western™: 1:50 - 1:250\nImmunoprecipitation: 1:100\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nFADS1 (F1D2A) Rabbit Monoclonal Antibody recognizes endogenous levels of total FADS1 protein. This antibody does not cross-react with FADS2 protein.\nSpecies Reactivity: Human, Mouse, Rat\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Phe100 of human FADS1 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nFatty acid desaturase 1 (FADS1), also known as delta-5 desaturase, is essential for the biosynthesis of highly unsaturated fatty acids (HUFAs) from linoleic acid (LA) and α-linolenic acid (ALA), both of which are polyunsaturated fatty acids (PUFAs). In addition, FADS1 converts NADH to NAD . Studies show that FADS1 and fatty acid desaturase 2 (FADS2) thus enable NAD recycling for glycolysis to support cell viability when aerobic respiration is acutely suppressed (1,2). Furthermore, FADS1 and ELOVL5 expression is enhanced in mesenchymal-type gastric cancer cells, sensitizing the cancer cells to ferroptosis. On the contrary, FADS1 and ELOVL5 expression is inhibited in intestinal-type gastric cancer cells, leading to resistance to ferroptosis (3).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nAcyl-CoA (8-3)-desaturase; D5D; delta-5 desaturase; delta-5 fatty acid desaturase; Delta(5) desaturase; Delta(5) fatty acid desaturase; FADS1; FADS6; FADSD5; Fatty acid desaturase 1; FLJ38956; FLJ90273; linoleoyl-CoA desaturase (delta-6-desaturase)-like 1; LLCDL1; TU12\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R\nSENSITIVITY: Endogenous\nMW (kDa): 43\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46797535707305,"sku":"10076T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-10076t","provider":"Iright","version":"1.0","type":"link"}