CST, 10208SF, DCLK1/DCAMKL1 (D2U3L) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying DCAMKL1. Validated for Western Blotting,Immunohistochemistry (Paraffin). Highly specific and rigorously validated in-house, DCLK1/DCAMKL1 (D2U3L) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #10208) is ready to ship. Product Usage Information This product is the carrier free version of product #62257. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 62257 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity DCLK1/DCAMKL1 (D2U3L) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total DCLK1/DCAMKL1 protein. The identity of the faint lower molecular weight bands is not known. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human DCLK1/DCAMKL1 protein. Background Doublecortin-like kinase 1 (DCLK1, DCAMKL1) is a serine/threonine kinase that belongs to the CaM kinase family and shares homology with the neuronal microtubule binding protein doublecortin. DCLK1 is thought to be involved in calcium signaling pathways controlling neuronal development in the embryonic brain (1,2). The kinase also functions in the mature nervous system and is highly expressed in regions of active neurogenesis in the neocortex and cerebellum (3,4). Research studies suggest that the DCLK1 kinase is highly expressed in subpopulations of cells within the colon and gastric epithelium and in the pancreas (5-8). The nature of these cell populations, whether normal, stem-like, or tumor-initiating, is unclear. Alternate Names CL1; CLICK1; DCAMKL1; DCDC3A; DCLK; DCLK1; doublecortin and CaM kinase-like 1; Doublecortin domain-containing protein 3A; doublecortin like kinase 1; Doublecortin-like and CAM kinase-like 1; Doublecortin-like kinase 1; KIAA0369; Serine/threonine-protein kinase DCLK1 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 82 Source/Isotype: Rabbit IgG