CST, 10334T, RBPMS (F5D2I) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying RBPMS. Validated for WB,IP,IF,IF. Available in 2 sizes. Highly specific and rigorously validated in-house, RBPMS (F5D2I) Rabbit Monoclonal Antibody (CST #10334) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunofluorescence (Frozen): 1:50 - 1:200 Immunofluorescence (Immunocytochemistry): 1:400 - 1:1600 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Frozen), Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity RBPMS (F5D2I) Rabbit Monoclonal Antibody recognizes endogenous levels of total RBPMS protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human RBPMS protein. Background RNA-binding protein with multiple splicing (RBPMS) is a member of the RNA recognition motif (RRM) family of proteins responsible for regulating gene expression and pre-mRNA alternative splicing events. RBPMS contains a single RRM domain at its amino terminus, which preferentially binds to tandem CAC trinucleotide motifs on target RNA (1). This protein is expressed in various tissues, with the highest levels detected in organs rich in smooth muscle, such as the heart, bladder, and gastrointestinal tract (2). RBPMS plays a critical role in normal heart development and cardiomyocyte binucleation by regulating RNA alternative splicing, and its loss or disruption results in contractility defects and dilated cardiomyopathy (3,4). Additionally, RBPMS serves as a selective marker for retinal ganglion cells (5). Alternate Names FLJ32971; Heart and RRM expressed sequence; HERMES; RBP-MS; RBPMS; RNA binding protein with multiple splicing; RNA binding protein, mRNA processing factor; RNA-binding protein with multiple splicing Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 24, 22 Source/Isotype: Rabbit IgG