{"product_id":"cst-10371s","title":"CST,  10371S, Glycogen Synthase 1 (GYS1) (F4L8X) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying Glycogen Synthase 1. Validated for Western Blotting,Simple Western™. Available in 2 sizes. Highly specific and rigorously validated in-house, Glycogen Synthase 1 (GYS1) (F4L8X) Rabbit Monoclonal Antibody (CST #10371) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nSimple Western™: 1:50 - 1:250\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nGlycogen Synthase 1 (GYS1) (F4L8X) Rabbit Monoclonal Antibody recognizes endogenous levels of total GYS1 protein. This antibody does not cross-react with GYS2 protein.\nSpecies Reactivity: Human, Mouse, Rat\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human GYS1 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nGlycogen is a polysaccharide of glucose and serves as an energy storage in mammalian muscle and liver (1). Glycogen synthase catalyzes the rate-limiting step of glycogen biosynthesis and has two major isoforms in mammals: muscle isoform (glycogen synthase 1, GYS1) and liver isoform (glycogen synthase 2, GYS2), respectively (1). Glycogen synthase kinase-3α (GSK-3α) and glycogen synthase kinase-3β (GSK-3β) phosphorylate glycogen synthase at multiple sites in its C-terminus (Ser641, Ser645, Ser649, and Ser653), inhibiting its activity (2,3). Hypoxia alters glycogen metabolism including temporal changes of GYS1 expression and phosphorylation in cancer cells, suggesting the role of metabolic reprogramming of glycogen metabolism in cancer growth (1).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nGlycogen [starch] synthase, muscle; glycogen synthase 1; glycogen synthase 1 (muscle); GSY; GYS; GYS1\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R\nSENSITIVITY: Endogenous\nMW (kDa): 84\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46797536395433,"sku":"10371S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-10371s","provider":"Iright","version":"1.0","type":"link"}