CST, 10424S, G-CSF-R (E8E7D) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying GCSFR. Validated for Western Blotting,Immunoprecipitation,Flow Cytometry (Live). Available in 2 sizes. Highly specific and rigorously validated in-house, G-CSF-R (E8E7D) Rabbit Monoclonal Antibody (CST #10424) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Flow Cytometry (Live): 1:50 - 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Flow Cytometry (Live) Specificity / Sensitivity G-CSF-R (E8E7D) Rabbit Monoclonal Antibody recognizes endogenous levels of total G-CSF-R protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the extracellular domain of human G-CSF-R protein. Background Granulocyte colony-stimulating factor receptor (G-CSF-R) is a transmembrane protein composed of an immunoglobulin-like (Ig-like) domain, a cytokine receptor-homologous (CRH) domain, and three fibronectin type III (FNIII) domains (1). G-CSF-R is expressed on all granulocytic lineages, including progenitor cells, and has been detected on monocytes, T and B lymphocytes, as well as non-hematopoietic tissues, including cardiomyocytes and neural stem cells (2-6). The primary ligand for G-CSF-R is the cytokine granulocyte colony-stimulating factor (G-CSF). G-CSF-R has no intrinsic tyrosine kinase activity; ligand binding induces conformational changes in the receptor, leading to activation of the Jak/Stat, PI3K/Akt, and MAPK pathways (7-10). G-CSF induces differentiation and proliferation of myeloid progenitor cells into neutrophils (11). Multiple diseases have been associated with mutations of the G-CSF-R gene, , including severe congenital neutropenia (SCN), chronic neutrophilic leukemia (CNL), and atypical chronic myeloid leukemia (aCML) (12). Alternate Names CD114; CD114 antigen; colony stimulating factor 3 receptor; colony stimulating factor 3 receptor (granulocyte); CSF3R; G-CSF receptor; G-CSF-R; GCSFR; Granulocyte colony-stimulating factor receptor; SCN7 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 50-80, 110-140 Source/Isotype: Rabbit IgG