{"product_id":"cst-10496s","title":"CST,  10496S, Phospho-SMAD3 (Ser423\/425) Matched Antibody Pair","description":"Matched Antibody Pair for studying Smad3 (Ser423\/Ser425) phosphate in the research area.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nMatched Antibody Pairs consist of capture and detection antibodies that bind to non-overlapping epitopes. For specific identification of the capture and detection antibodies in this pair, please refer to the data figure caption. Optimal dilutions\/concentrations should be determined by the end user.\n\u003cb\u003eFormulation\u003c\/b\u003e\nSupplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free.\n\u003cb\u003eStorage\u003c\/b\u003e\nStore at -20ºC. This product will freeze at -20ºC so it is recommended to aliquot into single-use vials to avoid multiple freeze\/thaw cycles . A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.\n\u003cb\u003eBackground\u003c\/b\u003e\nMembers of the SMAD family of signal transduction molecules are components of a critical intracellular pathway that transmit TGF-β signals from the cell surface into the nucleus. Three distinct classes of SMADs have been defined: the receptor-regulated SMADs (R-SMADs), which include SMAD1, 2, 3, 5, and 9; the common-mediator SMAD (co-SMAD), SMAD4; and the antagonistic or inhibitory SMADs (I-SMADs), SMAD6 and 7 (1-5). Activated type I receptors associate with specific R-SMADs and phosphorylate them on a conserved carboxy-terminal SSXS motif. The phosphorylated R-SMADs dissociate from the receptor and form a heteromeric complex with SMAD4, initiating translocation of the heteromeric SMAD complex to the nucleus. Once in the nucleus, SMADs recruit a variety of DNA binding proteins that function to regulate transcriptional activity (6-8).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nDKFZp586N0721; DKFZp686J10186; hMAD-3; hSMAD3; HSPC193; HsT17436; JV15-2; LDS1C; LDS3; MAD homolog 3; mad homolog JV15-2; mad protein homolog; MAD, mothers against decapentaplegic homolog 3; Mad3; MADH3; MGC60396; Mothers against decapentaplegic homolog 3; Mothers against DPP homolog 3; SMA- and MAD-related protein 3; SMAD 3; SMAD family member 3; SMAD, mothers against DPP homolog 3; SMAD3\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M Mi","brand":"CST","offers":[{"title":"Default Title","offer_id":46800303587497,"sku":"10496S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-10496s","provider":"Iright","version":"1.0","type":"link"}