CST, 10650T, NLRP6 (E2S8A) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying NLRP6. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, NLRP6 (E2S8A) Rabbit Monoclonal Antibody (CST #10650) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity NLRP6 (E2S8A) Rabbit Monoclonal Antibody recognizes endogenous levels of total NLRP6 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human NLRP6 protein. Background NLR family pyrin domain containing 6 (NLRP6, NALP6, PYPAF5), a member of the NOD-like receptor (NLR) family, acts as an innate immune sensor of microbe-associated molecular patterns (1). NLRP6 is primarily expressed in epithelial cells, mainly in the gastrointestinal tract (1,2,5). Like other NLR family members, NLRP6 is composed of three domains: a pyrin domain (PYD), a nucleotide-binding domain (NBD), and a leucine-rich repeat (LRR) domain (5). Upon activation, NLRP6 may form an inflammasome, a multiprotein complex that functions as a sensor of damage-associated molecular patterns (1-5). The NLRP6 inflammasome is involved in the activation of caspase-1 and subsequent processing of pro-inflammatory cytokines IL-1β and IL-18 as part of the innate immune response (1-5). NLRP6 may also play a role in regulating intestinal homeostasis (2-5). Alternate Names Angiotensin II/vasopressin receptor; AVR; CLR11.4; NACHT, leucine rich repeat and PYD containing 6; NACHT, LRR and PYD containing protein 6; NACHT, LRR and PYD domains-containing protein 6; NALP6; NAVR; NAVR/AVR; NLR family pyrin domain containing 6; NLR family, pyrin domain containing 6; NLRP6; nucleotide-binding oligomerization domain, leucine rich repeat and pyrin domain containing 6; PAN3; PYPAF5; PYRIN-containing APAF1-like protein 5 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 99 Source/Isotype: Rabbit IgG