CST, 10666S, MRP6/ABCC6 (D9D1F) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying MRP6. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, MRP6/ABCC6 (D9D1F) Rabbit Monoclonal Antibody (CST #10666) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity MRP6/ABCC6 (D9D1F) Rabbit Monoclonal Antibody recognizes endogenous levels of total MRP6 protein. This antibody also cross-reacts with unidentified proteins of 65 kDa and 135 kDa. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human MRP6 protein. Background The multidrug resistance-associated protein 6 (MRP6, ABCC6) is a member of ATP-binding cassette (ABC) family transporters that move drugs and hydrophobic compounds across cell membranes. The MRP6 protein is expressed mainly in liver and kidney, and in other tissues to a lesser extent (1). Identified MRP6 substrates include the glutathione conjugate of N-ethylmaleimide (NEM-GS) and leukotriene C4 (LTC4), with more tentative MRP6 substrates under investigation (2,3). Research studies show that increased MRP6 expression correlates with induced cholesterol biosynthesis, which suggests that MRP6 may be involved in lipid and cholesterol homeostasis (4). A small isoform of MRP6 is up-regulated in HBV infected hepatocytes and protects the cells from apoptosis mediated by caspase 3 and caspase 8 (5,6). Mutations in the corresponding gene cause pseudoxanthoma elasticum (PXE), an autosomal recessive disorder that is characterized by the accumulation of mineralized and fragmented elastic fibers in the skin, eyes, and arteries (7,8). Mutations in also result in generalized arterial calcification of infancy, an ectopic calcification disease that lies along a spectrum of similar disorders with PXE (9). Alternate Names ABC34; ABCC6; Anthracycline resistance-associated protein; ARA; ATP binding cassette subfamily C member 6; ATP-binding cassette sub-family C member 6; ATP-binding cassette, sub-family C (CFTR/MRP), member 6; EST349056; GACI2; MLP1; MOAT-E; MOATE; MRP6; Multi-specific organic anion transporter E; Multidrug resistance-associated protein 6; PXE; PXE1; URG7; URG7 protein Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 160-200 Source/Isotype: Rabbit IgG