{"product_id":"cst-10806s","title":"CST,  10806S, BORIS (F6R3W) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying BORIS. Validated for WB,WB,IP,IF,F,ChIP. Available in 2 sizes. Highly specific and rigorously validated in-house, BORIS (F6R3W) Rabbit Monoclonal Antibody (CST #10806) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nFor optimal ChIP results, use 10 μL of antibody and 10 μg of chromatin (approximately 4 × 10 6 cells) per IP. This antibody has been validated using SimpleChIP ®  Enzymatic Chromatin IP Kits.\nWestern Blotting: 1:1000\nSimple Western™: 1:10 - 1:50\nImmunoprecipitation: 1:100\nImmunofluorescence (Immunocytochemistry): 1:400 - 1:1600\nFlow Cytometry (Fixed\/Permeabilized): 1:200 - 1:800\nChromatin IP: 1:50\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed\/Permeabilized), Chromatin IP\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nBORIS (F6R3W) Rabbit Monoclonal Antibody recognizes endogenous levels of total BORIS protein. This antibody is predicted to detect isoforms 1, 7, and 8. This antibody detects a band of approximately 70 kDa that is predicted to be a BORIS isoform or cleavage product.\nSpecies Reactivity: Human\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro629 of human BORIS protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nBrother of the Regulator of Imprinted Sites (BORIS), also known as CCCTC-binding factor like (CTCFL), is a DNA-binding protein that functions as a transcriptional modulator. Similar to CCCTC-binding factor (CTCF), BORIS plays critical roles in chromatin organization and is thought to impact the chromatin landscape by interfering with CTCF-mediated chromatin loops (1,2). BORIS can also act as a transcriptional coactivator by recruiting other activators and chromatin-modifying proteins (3-5). Unlike CTCF, which is expressed uniformly in both healthy and cancerous tissues, BORIS expression is normally restricted to the testis and embryonic stem cells (6,7). However, BORIS is aberrantly expressed in various types of cancer and it is associated with high-risk features that include resistance to treatment (8-11).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nBORIS; BORIS-like protein; Brother of the regulator of imprinted sites; Cancer\/testis antigen 27; CCCTC-binding factor; CCCTC-binding factor (zinc finger protein)-like; CCCTC-binding factor like; CT27; CTCF paralog; CTCF-like protein; CTCF-T; CTCFL; dJ579F20.2; HMG-1L1; HMGB1L1; MGC163358; MGC169105; MGC169106; putative high mobility group protein 1-like 1; putative high mobility group protein B1-like 1; Transcriptional repressor CTCFL; Zinc finger protein CTCF-T\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H\nSENSITIVITY: Endogenous\nMW (kDa): 90\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46797548028073,"sku":"10806S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-10806s","provider":"Iright","version":"1.0","type":"link"}