CST, 10806T, BORIS (F6R3W) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying BORIS. Validated for WB,WB,IP,IF,F,ChIP. Available in 2 sizes. Highly specific and rigorously validated in-house, BORIS (F6R3W) Rabbit Monoclonal Antibody (CST #10806) is ready to ship. Product Usage Information For optimal ChIP results, use 10 μL of antibody and 10 μg of chromatin (approximately 4 × 10 6 cells) per IP. This antibody has been validated using SimpleChIP ®  Enzymatic Chromatin IP Kits. Western Blotting: 1:1000 Simple Western™: 1:10 - 1:50 Immunoprecipitation: 1:100 Immunofluorescence (Immunocytochemistry): 1:400 - 1:1600 Flow Cytometry (Fixed/Permeabilized): 1:200 - 1:800 Chromatin IP: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized), Chromatin IP Specificity / Sensitivity BORIS (F6R3W) Rabbit Monoclonal Antibody recognizes endogenous levels of total BORIS protein. This antibody is predicted to detect isoforms 1, 7, and 8. This antibody detects a band of approximately 70 kDa that is predicted to be a BORIS isoform or cleavage product. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro629 of human BORIS protein. Background Brother of the Regulator of Imprinted Sites (BORIS), also known as CCCTC-binding factor like (CTCFL), is a DNA-binding protein that functions as a transcriptional modulator. Similar to CCCTC-binding factor (CTCF), BORIS plays critical roles in chromatin organization and is thought to impact the chromatin landscape by interfering with CTCF-mediated chromatin loops (1,2). BORIS can also act as a transcriptional coactivator by recruiting other activators and chromatin-modifying proteins (3-5). Unlike CTCF, which is expressed uniformly in both healthy and cancerous tissues, BORIS expression is normally restricted to the testis and embryonic stem cells (6,7). However, BORIS is aberrantly expressed in various types of cancer and it is associated with high-risk features that include resistance to treatment (8-11). Alternate Names BORIS; BORIS-like protein; Brother of the regulator of imprinted sites; Cancer/testis antigen 27; CCCTC-binding factor; CCCTC-binding factor (zinc finger protein)-like; CCCTC-binding factor like; CT27; CTCF paralog; CTCF-like protein; CTCF-T; CTCFL; dJ579F20.2; HMG-1L1; HMGB1L1; MGC163358; MGC169105; MGC169106; putative high mobility group protein 1-like 1; putative high mobility group protein B1-like 1; Transcriptional repressor CTCFL; Zinc finger protein CTCF-T Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 90 Source/Isotype: Rabbit IgG