{"product_id":"cst-10923s","title":"CST,  10923S, NAGK (F9C7G) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying NAGK. Validated for Western Blotting,Simple Western™. Available in 2 sizes. Highly specific and rigorously validated in-house, NAGK (F9C7G) Rabbit Monoclonal Antibody (CST #10923) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nSimple Western™: 1:10 - 1:50\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nNAGK (F9C7G) Rabbit Monoclonal Antibody recognizes endogenous levels of total NAGK protein.\nSpecies Reactivity: Human, Mouse, Rat\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu65 of human NAGK protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nN-acetyl-D-glucosamine kinase (NAGK) is a member of the sugar kinase\/Hsp70\/actin superfamily, whose members phosphorylate certain substrates in an ATP-dependent manner (1-3). NAGK mediates the phosphorylation of N-acetylglucosamine (GlcNAc) to GlcNAc-6-phosphate, which is then used for biosynthesis of UDP-GlcNAc, a critical component of O-linked-N-acetylglucosaminylation and N-linked glycosylation (1-4). NAGK is expressed mainly in immune cells and is co-expressed with genes primarily associated with immune cell functions (4). NAGK can also phosphorylate muramyl dipeptide (MDP), triggering pro-inflammatory gene expression by NOD2 (nucleotide-binding oligomerization domain-containing protein), which is critical for maintaining a healthy intestinal barrier function (4).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nepididymis secretory sperm binding protein; GlcNAc kinase; GNK; HSA242910; N-acetyl-D-glucosamine kinase; N-acetylglucosamine kinase; NAGK\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R\nSENSITIVITY: Endogenous\nMW (kDa): 37\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46797551730857,"sku":"10923S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-10923s","provider":"Iright","version":"1.0","type":"link"}