CST, 10962S, FAT1 Antibody

Polyclonal Antibody for studying FAT1. Validated for Western Blotting. Highly specific and rigorously validated in-house, FAT1 Antibody (CST #10962) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity FAT1 Antibody recognizes endogenous levels of total FAT1 protein. Species Reactivity: Human, Mouse Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human FAT1 protein. Antibodies are purified by peptide affinity chromatography. Background FAT1 is a member of the FAT atypical cadherin (FAT) subfamily of cadherin proteins (1). FAT1 is a single-pass transmembrane protein, first identified in a screen for tumor suppressor proteins in (2). FAT1 is expressed primarily in epithelial cells, where it plays a prominent role in regulating cell growth and migration, in large part through the regulation of cell-cell adhesion dynamics (3). The intracellular cytoplasmic tail of FAT1 contains multiple functional motif/domains that regulate FAT1 functions, including a proline rich EVH1 binding motif that regulates actin cytoskeleton components (e.g., Ena/VASP proteins) at both cell-cell contact points and leading edges of migrating cells (4,5). FAT1 appears to play a role in linking cell adhesion events to intracellular signaling pathways. For example, FAT1 was capable of inhibiting the nuclear translocation of β-catenin through its cytoplasmic FC1 domain interaction with β-catenin (6), and activating the Hippo signaling pathway, suppressing YAP signaling by its N-terminal cytoplasmic region interaction with MST1 (7). Research studies have revealed that the tumor suppressor functions identified in are conserved in vertebrate FAT1 homologs (8). For example, studies in human cancer cells showed that loss-of-function mutations in the gene encoding FAT1 promoted a hybrid epithelial-to-mesenchymal transition, which further enabled the development of cancer drug resistance (9). Notably, studies have also revealed an oncogenic function for FAT1 in some contexts (10). Alternate Names Cadherin family member 7; cadherin ME5; cadherin-related family member 8; Cadherin-related tumor suppressor homolog; CDHF7; CDHR8; FAT; FAT atypical cadherin 1; FAT tumor suppressor 1; FAT tumor suppressor homolog 1 (Drosophila); FAT1; hFat1; ME5; Protein fat homolog; Protocadherin Fat 1; Protocadherin Fat 1, nuclear form Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 550 SOURCE: Rabbit