{"product_id":"cst-11815s","title":"CST,  11815S, ATF-4 (D4B8) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying ATF-4. Validated for WB,IP,IF,ChIP,ChIP,C\u0026amp;R,C\u0026amp;T. Available in 3 sizes. Highly specific and rigorously validated in-house, ATF-4 (D4B8) Rabbit Monoclonal Antibody (CST #11815) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nFor optimal ChIP and ChIP-seq results, use 2.5 μl of antibody and 10 μg of chromatin (approximately 4 x 10 6 cells) per IP. This antibody has been validated using SimpleChIP ® Enzymatic Chromatin IP Kits. The CUT\u0026amp;RUN dilution was determined using the CUT\u0026amp;RUN Assay Kit # 86652 . The CUT\u0026amp;Tag dilution was determined using CUT\u0026amp;Tag Assay Kit # 77552 .\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:50\nImmunofluorescence (Immunocytochemistry): 1:100 - 1:400\nChromatin IP: 1:200\nChromatin IP-seq: 1:200\nCUT\u0026amp;RUN: 1:200\nCUT\u0026amp;Tag: 1:200\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 82093 .\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry), Chromatin IP, Chromatin IP-seq, CUT\u0026amp;RUN, CUT\u0026amp;Tag\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nATF-4 (D4B8) Rabbit Monoclonal Antibody recognizes endogenous levels of total ATF-4 protein. Reactivity by immunofluorescence is human only.\nSpecies Reactivity: Human, Mouse, Rat\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human ATF-4 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nATF-4, an activating transcription factor\/cAMP-response element-binding protein family member, functions in the PERK and eIF2α ER stress responsive pathway (1-3). ER stress represses the translation of the majority of mRNAs, but selectively stimulates the translation of certain mRNAs including that of ATF-4 (2). Induced expression of ATF-4 increases the expression of genes critical for the recovery from ER stress (4).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nActivating transcription factor 4; activating transcription factor 4 (tax-responsive enhancer element B67); ATF-4; ATF4; cAMP response element-binding protein 2; cAMP-dependent transcription factor ATF-4; cAMP-responsive element-binding protein 2; CREB-2; CREB2; Cyclic AMP-dependent transcription factor ATF-4; Cyclic AMP-responsive element-binding protein 2; DNA-binding protein TAXREB67; tax-responsive enhancer element B67; Tax-responsive enhancer element-binding protein 67; TaxREB67; TXREB\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R\nSENSITIVITY: Endogenous\nMW (kDa): 49\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46797552779433,"sku":"11815S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-11815s","provider":"Iright","version":"1.0","type":"link"}