CST, 11828S, Arrestin 1/S-Arrestin Antibody

Polyclonal Antibody for studying Rod Arrestin. Validated for Western Blotting. Highly specific and rigorously validated in-house, Arrestin 1/S-Arrestin Antibody (CST #11828) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Arrestin 1/S-Arrestin Antibody recognizes endogenous levels of total Arrestin 1/S-Arrestin protein. Species Reactivity: Human, Mouse, Rat Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala163 of human Arrestin 1/S-Arrestin protein. Antibodies are purified by protein A and peptide affinity chromatography. Background Arrestin proteins function as negative regulators of G protein-coupled receptor (GPCR) signaling. Cognate ligand binding stimulates GPCR phosphorylation, which is followed by binding of arrestin to the phosphorylated GPCR and the eventual internalization of the receptor and desensitization of GPCR signaling (1). Four distinct mammalian arrestin proteins are known. Arrestin 1 (also known as S-arrestin) and arrestin 4 (X-arrestin) are localized to retinal rods and cones, respectively. Arrestin 2 (also known as β-arrestin 1) and arrestin 3 (β-arrestin 2) are ubiquitously expressed and bind to most GPCRs (2). β-arrestins function as adaptor and scaffold proteins and play important roles in other processes, such as recruiting c-Src family proteins to GPCRs in Erk activation pathways (3,4). β-arrestins are also involved in some receptor tyrosine kinase signaling pathways (5-8). Additional evidence suggests that β-arrestins translocate to the nucleus and help regulate transcription by binding transcriptional cofactors (9,10). Arrestin 1/S-Arrestin inactivates rhodopsin-mediated signaling through binding to the light-activated phosphorylated rod photoreceptor, thereby preventing coupling to transducin, rhodopsin's cognate G protein (11). Research studies have proposed that mutations in the Arrestin 1/S-Arrestin gene are linked to Oguchi disease (12,13) and Retinitis Pigmentosa (14). Alternate Names 48 kDa protein; arrestin 1; ARRS; DKFZp686D1084; DKFZp686I1383; retina and pineal gland (arrestin); Retinal S-antigen; retinal S-antigen (48 KDa protein); rod arrestin; Rod photoreceptor arrestin; RP47; S-AG; S-antigen; S-antigen visual arrestin; S-arrestin; SAG Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 52 SOURCE: Rabbit