CST, 11875S, UPF2 (D3B10) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying UPF2. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, UPF2 (D3B10) Rabbit Monoclonal Antibody (CST #11875) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity UPF2 (D3B10) Rabbit Monoclonal Antibody recognizes endogenous levels of total UPF2 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu391 of human UPF2 protein. Background UPF1 was identified as an active component in nonsense-mediated mRNA decay (NMD), an mRNA surveillance mechanism in eukaryotic cells that degrades mRNAs containing premature termination codons (1). UPF1 was found to be an ATP-dependent RNA helicase in the cytoplasm (2) and was later shown to be a component of cytoplasmic P-bodies (3). UPF1 phosphorylation mediates the repression of translation that accompanies NMD, allowing mRNA accessibility to the NMD machinery (4). Two other active components of NMD, UPF2 and UPF3, were also identified and described as having perinuclear and nucleocytoplasmic localization, respectively (5). Alternate Names DKFZp434D222; FRS2/UPF2/LEMD3 fusion; hUpf2; KIAA1408; LEMD3/UPF2 fusion; MGC138834; MGC138835; Nonsense mRNA reducing factor 2; Regulator of nonsense transcripts 2; RENT2; smg-3; smg-3 homolog, nonsense mediated mRNA decay factor; Up-frameshift suppressor 2 homolog; UPF2; UPF2 regulator of nonsense mediated mRNA decay; UPF2 regulator of nonsense transcripts homolog; UPF2 regulator of nonsense transcripts homolog (yeast); yeast Upf2p homolog Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 170 Source/Isotype: Rabbit IgG