CST, 11890S, PINCH (5G7) Mouse Monoclonal Antibody

Monoclonal Antibody for studying LIMS1. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, PINCH (5G7) Mouse Monoclonal Antibody (CST #11890) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity PINCH (5G7) Mouse Monoclonal Antibody recognizes endogenous levels of total PINCH protein. This antibody recognizes a protein of unknown origin at 80 kDa in some cell lines. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a recombinant protein specific to the the human PINCH protein. Background The extracellular matrix (ECM) is a complex structure of secreted macromolecules surrounding mammalian organs and tissues. Controlled interactions between cells and the ECM are important in proliferation, migration, survival, polarity, and differentiation. Cells contact the ECM primarily through focal adhesion complexes, which contain integrins, as well as multiple adaptor and signaling proteins (1). The ILK/PINCH/Parvin (IPP) adaptor complex acts at the interface of the integrin/actin connection to regulate formation of focal adhesions and integrin signaling. Roles for IPP proteins outside of the IPP complex have been proposed, including regulation of gene expression (2,3). PINCH, also known as LIMS1, has been shown to function as a specific regulator of gene expression in glomerular podocytes in response to TGF-β1 (4). Researchers have shown that PINCH is highly expressed in some human tumors, and that PINCH can promote resistance to ionizing radiation through activation of Akt (5,6). Alternate Names LIM and senescent cell antigen-like domains 1; LIM and senescent cell antigen-like-containing domain protein 1; LIM zinc finger domain containing 1; LIM-type zinc finger domains 1; LIMS1; Particularly interesting new Cys-His protein 1; PINCH; PINCH-1; PINCH1; Renal carcinoma antigen NY-REN-48; senescent cell antigen Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 35 Source/Isotype: Mouse IgG1