{"product_id":"cst-11892s","title":"CST,  11892S, Phospho-HSP27 (Ser82) (D1H2F6) Rabbit Monoclonal Antibody (Alexa Fluor® 647 Conjugate)","description":"Monoclonal Antibody for studying HSP27 (Ser82) phosphate. Validated for Flow Cytometry (Fixed\/Permeabilized). Highly specific and rigorously validated in-house, Phospho-HSP27 (Ser82) (D1H2F6) Rabbit Monoclonal Antibody (Alexa Fluor\u003csup\u003e®\u003c\/sup\u003e 647 Conjugate) (CST #11892) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nFlow Cytometry (Fixed\/Permeabilized): 1:50\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg\/ml BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Flow Cytometry (Fixed\/Permeabilized)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nPhospho-HSP27 (Ser82) (D1H2F6) Rabbit Monoclonal Antibody (Alexa Fluor\nSpecies Reactivity: Human, Mouse\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser82 of human HSP27 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nHeat shock protein (HSP) 27 is one of the small HSPs that are constitutively expressed at different levels in various cell types and tissues. Like other small HSPs, HSP27 is regulated at both the transcriptional and posttranslational levels (1). In response to stress, the HSP27 expression increases several-fold to confer cellular resistance to the adverse environmental change. HSP27 is phosphorylated at Ser15, Ser78, and Ser82 by MAPKAPK-2 as a result of the activation of the p38 MAP kinase pathway (2,3). Phosphorylation of HSP27 causes a change in its tertiary structure, which shifts from large homotypic multimers to dimers and monomers (4). It has been shown that phosphorylation and increased concentration of HSP27 modulates actin polymerization and reorganization (5,6).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\n28 kDa heat shock protein; CMT2F; DKFZp586P1322; epididymis secretory protein Li 102; Estrogen-regulated 24 kDa protein; Heat shock 27 kDa protein; heat shock 27kD protein 1; heat shock 27kDa protein 1; Heat shock protein beta-1; heat shock protein family B (small) member 1; HEL-S-102; HMN2B; HS.76067; HSP 27; Hsp25; HSP27; HSP28; HSPB1; SRP27; Stress-responsive protein 27\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M\nSENSITIVITY: Endogenous\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46800335470761,"sku":"11892S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-11892s","provider":"Iright","version":"1.0","type":"link"}