CST, 11921S, DYRK2 (D9A3K) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying DYRK2. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, DYRK2 (D9A3K) Rabbit Monoclonal Antibody (CST #11921) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity DYRK2 (D9A3K) Rabbit Monoclonal Antibody recognizes endogenous levels of total DYRK2 protein. This antibody recognizes both known isoforms, 66 and 60 kDa, of DYRK2. Species Reactivity: Human, Mouse, Rat, Hamster, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly545 of human DYRK2 protein. Background The DYRK family includes several ual-specificity t rosine-phosphorylated and egulated inases capable of phosphorylating proteins at both Tyr and Ser/Thr residues (1). The DYRK family was identified based on homology to the yeast Yak1 (2) and the minibrain (mnb) kinases (3). Seven mammalian isoforms have been discovered, including DYRK1A, DYRK1B, DYRK1C, DYRK2, DYRK3, DYRK4, and DYRK4B. Differences in substrate specificity, expression, and subcellular localization are seen across the DYRK family (4,5). All DYRK proteins have a Tyr-X-Tyr motif in the catalytic domain activation loop; phosphorylation of the second Tyr residue (e.g. Tyr312 of DYRK1A) is necessary for kinase activity. DYRKs typically autophosphorylate the Tyr residue within their activation loop, but phosphorylate substrates at Ser and Thr residues (1,6). DYRK2 is thought to play a role in checkpoint control of the cell cycle. DYRK2 can phosphorylate p53 at Ser46 following cellular damage, leading to activation of the apoptotic response (7). Research studies have demonstrated overexpression of DYRK2 in esophageal and lung adenocarcinomas (8), with DYRK2 expression levels acting as a potential predictor of chemotherapy treatment outcomes in non-small cell lung cancer (9). Alternate Names dual specificity tyrosine phosphorylation regulated kinase 2; dual specificity tyrosine-(Y)-phosphorylation regulated kinase 2; Dual specificity tyrosine-phosphorylation-regulated kinase 2; dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 2; DYRK2; FLJ21217; FLJ21365 Specification REACTIVITY: H M R Hm Mk SENSITIVITY: Endogenous MW (kDa): 60, 66 Source/Isotype: Rabbit IgG