{"product_id":"cst-11933s","title":"CST,  11933S, CAD Antibody","description":"Polyclonal Antibody for studying CAD. Validated for Western Blotting. Highly specific and rigorously validated in-house, CAD Antibody (CST #11933) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nCAD Antibody recognizes endogenous levels of total CAD protein. This antibody may cross-react with a protein of unknown origin at ~45 kDa in some cell lines.\nSpecies Reactivity: Human, Mouse, Rat\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nPolyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human CAD protein. Antibodies are purified by protein A and peptide affinity chromatography.\n\u003cb\u003eBackground\u003c\/b\u003e\nCAD is essential for the synthesis of pyrimidine nucleotides and possesses the following enzymatic activities: glutamine amidotransferase, carbamoyl-phosphate synthetase, aspartate transcarbamoylase, and dihydroorotase. Thus, the enzyme converts glutamine to uridine monophosphate, a common precursor of all pyrimidine bases, and it is necessary for nucleic acid synthesis (1). In resting cells, CAD is localized mainly in the cytoplasm where it carries out pyrimidine synthesis. As proliferating cells enter S phase, MAP Kinase (Erk1\/2) phosphorlyates CAD at Thr456, resulting in CAD translocation to the nucleus. As cells exit S phase, CAD is dephosphorylated at Thr456 and phosphorylated at Ser1406 by PKA, returning the pathway to basal activity (2). Various research studies have shown increased expression of CAD in several types of cancer, prompting the development of pharmacological inhibitors such as PALA. Further studies have identified CAD as a potential predictive early marker of prostate cancer relapse (3).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nAspartate carbamoyltransferase; CAD; CAD protein; CAD trifunctional protein; carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase; CDG1Z; Dihydroorotase; EIEE50; GATD4; Glutamine-dependent carbamoyl-phosphate synthase; multifunctional protein CAD; PYR1\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R\nSENSITIVITY: Endogenous\nMW (kDa): 240\nSOURCE: Rabbit","brand":"CST","offers":[{"title":"Default Title","offer_id":46797561921705,"sku":"11933S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-11933s","provider":"Iright","version":"1.0","type":"link"}