CST, 11973S, CDK12 Antibody

Polyclonal Antibody for studying CDK12. Validated for Western Blotting. Highly specific and rigorously validated in-house, CDK12 Antibody (CST #11973) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity CDK12 Antibody recognizes endogenous levels of total CDK12 protein. This antibody also cross-reacts with proteins of unknown origin at 48 and 53 kDa. Species Reactivity: Human, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val492 of human CDK12 protein. Antibodies are purified by protein A and peptide affinity chromatography. Background Cyclin-dependent kinase 12 (CDK12/CRKRS/CRK7) is composed of a central CTD kinase domain, several proline-rich regions, and several amino-terminal arginine/serine (RS) motifs common to splicing factors (1). CDK12 is ubiquitously expressed and forms a complex with cyclin K that regulates phosphorylation of the C-terminal domain (CTD) of RNA polymerase II (1-4). CDK12 is important for expression of a subset of long genes with high numbers of exons including some regulators of the DNA damage response, such as breast and ovarian cancer type 1 susceptibility protein 1 (BRCA1) and ataxia telangiectasia and Rad3-related (ATR) (3). Depletion of CDK12 results in spontaneous DNA damage and increased sensitivity to DNA damage agents (3). Research studies have shown that CDK12 is recurrently mutated in high-grade ovarian cancer (5,6). In addition, high levels of CDK12 are required to maintain pluripotency of embryonic stem cells (7). Alternate Names Cdc2-related kinase, arginine/serine-rich; CDC2-related protein kinase 7; CDK12; Cell division cycle 2-related protein kinase 7; Cell division protein kinase 12; CRK7; CRKR; CRKRS; cyclin dependent kinase 12; Cyclin-dependent kinase 12; hCDK12; KIAA0904 Specification REACTIVITY: H Mk SENSITIVITY: Endogenous MW (kDa): 205 SOURCE: Rabbit