CST, 11983S, Phospho-BRIP1/FANCJ (Thr1133) Antibody

Polyclonal Antibody for studying BRIP1 (Thr1133) phosphate. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, Phospho-BRIP1/FANCJ (Thr1133) Antibody (CST #11983) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Phospho-BRIP1/FANCJ (Thr1133) Antibody recognizes endogenous levels of BRIP1/FANCJ protein only when phosphorylated at Thr1133. This antibody also cross-reacts with a protein of unknown origin at ~130 kDa. Species Reactivity: Human, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr1133 of human BRIP1/FANCJ protein. Antibodies are purified by protein A and peptide affinity chromatography. Background BACH1, also known as BRIP1 and FANCJ, is a DNA helicase involved in repairing DNA cross-links and double-strand breaks (1-3). Interaction between phosphorylated BACH1 and BRCA1 is required for DNA damage-induced checkpoint signaling (3,4). Originally identified as a breast cancer susceptibility gene (1), the gene is mutated in Fanconi anemia (5), a recessive disorder characterized by multiple congenital abnormalities, progressive bone marrow failure, and high cancer risk/predisposition. Research investigators have concluded that BACH1 interactions with BRCA1 and the presence of mutations in patients with early-onset breast cancer indicate that BACH1 may act as a tumor suppressor (6).BACH1 phosphorylation at Thr1133 is thought to be involved in regulating the replication checkpoint and is required for BACH1 interaction with TopBP1 (7). Alternate Names ATP-dependent RNA helicase BRIP1; BACH1; BRCA1 interacting protein C-terminal helicase 1; BRCA1-associated C-terminal helicase 1; BRCA1-binding helicase-like protein BACH1; BRCA1-interacting protein 1; BRCA1-interacting protein C-terminal helicase 1; BRCA1/BRCA2-associated helicase 1; BRIP1; FANCJ; Fanconi anemia group J protein; FLJ90232; MGC126521; MGC126523; OF; Protein FACJ Specification REACTIVITY: H Mk SENSITIVITY: Endogenous MW (kDa): 145 SOURCE: Rabbit