CST, 11992T, Phospho-MARCKS (Ser159/163) (D13D2) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying MARCKS (Ser159/Ser163) phosphate. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-MARCKS (Ser159/163) (D13D2) Rabbit Monoclonal Antibody (CST #11992) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Phospho-MARCKS (Ser159/163) (D13D2) Rabbit Monoclonal Antibody recognizes endogenous levels of MARCKS protein when phosphorylated at Ser159 and Ser163 (human residues). Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser159 and Ser163 of human MARCKS protein. Background yristoylated lanine- ich - inase ubstrate (MARCKS) is a major PKC substrate expressed in many cell types. MARCKS has been implicated in cell motility, cell adhesion, phagocytosis, membrane traffic, and mitogenesis (1). PKC phosphorylates Ser159, 163, 167, and 170 of MARCKS in response to growth factors and oxidative stress. Phosphorylation at these sites regulates the calcium/calmodulin binding and filamentous (F)-actin cross-linking activities of MARCKS (2-4). Phosphorylation by PKC also results in translocation of MARCKS from the plasma membrane to the cytoplasm (5). Alternate Names 80K-L; 80K-L protein; FLJ14368; FLJ90045; MACS; MARCKS; MARCS; myristoylated alanine rich protein kinase C substrate; Myristoylated alanine-rich C-kinase substrate; myristoylated alanine-rich protein kinase C substrate; myristoylated alanine-rich protein kinase C substrate (MARCKS, 80K-L); phosphomyristin; PKCSL; PRKCSL; Protein kinase C substrate, 80 kDa protein, light chain Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 75 (rodent), 80 (human) Source/Isotype: Rabbit IgG