CST, 11993S, Phospho-KIF1B (Ser1487) Antibody

Polyclonal Antibody for studying KIF1B (Ser1487) phosphate. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, Phospho-KIF1B (Ser1487) Antibody (CST #11993) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Phospho-KIF1B (Ser1487) Antibody recognizes endogenous levels of KIF1B protein only when phosphorylated at Ser1487. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser1487 of human KIF1B protein. Antibodies are purified by protein A and peptide affinity chromatography. Background Kinesin-like protein KIF1B is a member of the kinesin 3 family of C-kinesins that are characterized by a kinesin-motor domain in the carboxy-terminal region. As part of the general mechanism of kinesin-mediated cellular transport, C-kinesins are known to drive microtubule plus and minus end motilities (1-3). KIF1B is implicated in the transport of synaptic proteins to the cell periphery in neuronal cell axons by interaction with Rab3 guanine nucleotide exchange factor (3). Mitochondria are also often transported in axons by KIF1B (3-4). Quantitative mass spectrometry profiling of mitotic phosphorylation revealed putative phosphorylation sites of KIF1B (5). Phospho-KIF1B (Ser1487) Antibody is directed at a site identified by Cell Signaling Technology (CST) using PhosphoScan , CST's LC-MS/MS platform for modification site discovery. Phosphorylation at Ser1487 was discovered using an Akt substrate antibody. Please visit PhosphoSitePlus , CST's modification site knowledgebase, at www.phosphosite.org for more information. Alternate Names CMT2; CMT2A; CMT2A1; FLJ23699; HMSNII; KIAA0591; KIAA1448; KIF1B; kinesin family member 1B; kinesin superfamily protein KIF1B; Kinesin-like protein KIF1B; Klp; MGC134844; NBLST1 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 140, 200 SOURCE: Rabbit