CST, 12029S, TRXR2/TXNRD2 (3F2-E12-F10) Mouse Monoclonal Antibody

Monoclonal Antibody for studying TXNRD2. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, TRXR2/TXNRD2 (3F2-E12-F10) Mouse Monoclonal Antibody (CST #12029) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity TRXR2/TXNRD2 (3F2-E12-F10) Mouse Monoclonal Antibody recognizes endogenous levels of total TRXR2/TXNRD2 protein. Species Reactivity: Human, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a recombinant protein specific to the carboxy terminus of human TRXR2/TXNRD2 protein. Background Thioredoxin reductases are selenoproteins that are critical for maintaining cellular redox balance and eliminating reactive oxygen species (ROS) (1-4). There are three known mammalian thioredoxin reductases: TRXR1/TXNRD1 (cytosolic), TRXR2/TXNRD2 (mitochondrial), and TRXR3/TXNRD3 (testis-specific) (1). TRXR2 may function to protect against tumor necrosis factor-α (TNF-α)-mediated ROS generation (5). TRXR2 is critical for normal heart development and function (1,4). TRXR2 KO mice experience embryonic lethality, while mitochondrial dysfunction seen in heart-specific TRXR2 KO mice results in congestive heart failure (4). In humans, certain rare mutations in TRXR2 have been linked to dilated cardiomyopathy (1). A recent study in the EPIC-Heidelberg cohort shows that serum selenium levels, in concert with certain SNPs in TRXR2 and other selenoproteins, can influence prostate cancer risk (6). Alternate Names GCCD5; KIAA1652; Selenoprotein Z; SelZ; thioredoxin reductase 2; Thioredoxin reductase 2, mitochondrial; thioredoxin reductase 3; thioredoxin reductase beta; Thioredoxin reductase TR3; TR; TR-beta; TR3; TRXR2; TXNRD2 Specification REACTIVITY: H Mk SENSITIVITY: Endogenous MW (kDa): 56 Source/Isotype: Mouse IgG3