{"product_id":"cst-12094t","title":"CST,  12094T, HMGA1 (D4F8) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying HMGA1. Validated for Western Blotting,Immunohistochemistry (Paraffin),Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, HMGA1 (D4F8) Rabbit Monoclonal Antibody (CST #12094) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunohistochemistry (Paraffin): 1:2000\nImmunofluorescence (Immunocytochemistry): 1:200\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunohistochemistry (Paraffin), Immunofluorescence (Immunocytochemistry)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nHMGA1 (D4F8) Rabbit Monoclonal Antibody recognizes endogenous levels of total HMGA1 protein, isoforms 1a and 1b. Based on sequence homology, this antibody is not predicted to cross-react with HMGA2.\nSpecies Reactivity: Human, Monkey\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly68 of human HMGA1 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nHMGA1, formerly known as HMG-I\/Y, belongs to a family of high mobility group proteins that contain an AT-hook DNA binding domain. HMGA proteins are considered architectural transcription factors; they do not have direct transcriptional activation capacity, but instead regulate gene expression by changing DNA conformation through binding to AT-rich regions in the DNA and\/or direct interaction with other transcription factors (1,2). HMGA1 is highly expressed during embryogenesis and in embryonic stem cells, but not in fully differentiated adult tissues (2-4). Research studies have shown that HMGA1 is over-expressed in rapidly dividing neoplastic cells and a wide variety of aggressive cancers, including thyroid, colon, breast, pancreas, and prostate (2-4). Investigators have shown that forced expression of HMGA1 induces cellular transformation and an epithelial-to-mesenchymal transition (EMT), while inhibition of HMGA1 expression blocks anchorage-independent cell growth and proliferation of cancer cells, suggesting that HMGA1 contributes to carcinogenesis by inducing and maintaining a de-differentiated, highly proliferative cell state (5-8).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nhigh mobility group AT-hook 1; High mobility group AT-hook protein 1; High mobility group protein A1; High mobility group protein HMG-I\/HMG-Y; High mobility group protein R; high-mobility group (nonhistone chromosomal) protein isoforms I and Y; HMG-I(Y); HMG-R; HMGA1; HMGA1A; HMGIY; MGC12816; MGC4242; MGC4854; nonhistone chromosomal high-mobility group protein HMG-I\/HMG-Y\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H Mk\nSENSITIVITY: Endogenous\nMW (kDa): 18\nSource\/Isotype: Rabbit","brand":"CST","offers":[{"title":"Default Title","offer_id":46797565788329,"sku":"12094T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-12094t","provider":"Iright","version":"1.0","type":"link"}