CST, 12098T, PGAM1 (D3J9T) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying PGAM1. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, PGAM1 (D3J9T) Rabbit Monoclonal Antibody (CST #12098) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity PGAM1 (D3J9T) Rabbit Monoclonal Antibody recognizes endogenous levels of total PGAM1 protein. This antibody may cross react with overexpressed PGAM2 protein. Species Reactivity: Human, Mouse, Rat, Hamster, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human PGAM1 protein. Background Phosphoglycerate mutase (PGAM1) catalyzes the conversion of 3-phosphoglycerate to 2-phosphoglycerate during glycolysis (1-5). Research studies have shown increased PGAM1 expression in cancer (1-4) and mental disease (5). Specifically, PGAM1 was shown to be phosphorylated at His11 by phosphoenolpyruvate (PEP) in PKM2-expressing cells, suggesting a possible regulatory role for PGAM1 in actively proliferating cells via an alternative glycolytic pathway (1). Alternate Names BPG-dependent PGAM 1; epididymis secretory protein Li 35; HEL-S-35; PGAM-B; PGAM1; PGAMA; Phosphoglycerate mutase 1; phosphoglycerate mutase 1 (brain); phosphoglycerate mutase A, nonmuscle form; Phosphoglycerate mutase isozyme B Specification REACTIVITY: H M R Hm Mk SENSITIVITY: Endogenous MW (kDa): 28 Source/Isotype: Rabbit IgG