CST, 12103S, SGK1 (D27C11) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying SGK1. Validated for Western Blotting,Simple Western™,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, SGK1 (D27C11) Rabbit Monoclonal Antibody (CST #12103) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:10 - 1:50 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity SGK1 (D27C11) Rabbit Monoclonal Antibody recognizes endogenous levels of total SGK1 protein. This antibody does not cross-react with SGK2 or SGK3. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly420 of human SGK1 protein. Background Serum and glucocorticoid-inducible kinase (SGK) is a serine/threonine kinase closely related to Akt (1). SGK is rapidly induced in response to a variety of stimuli, including serum, glucocorticoid, follicle stimulating hormone, osmotic shock, and mineralocorticoids. SGK activation can be accomplished via HGF PI3K-dependent pathways and by integrin-mediated PI3K-independent pathways (2,3). Induction and activation of SGK has been implicated in activating the modulation of anti-apoptotic and cell cycle regulation (4-6). SGK also plays an important role in activating certain potassium, sodium, and chloride channels, suggesting its involvement in the regulation of processes such as cell survival, neuronal excitability, and renal sodium excretion (2). SGK is negatively regulated by ubiquitination and proteasome degradation (7). In addition to its membrane channel and carrier functions, SGK1 also regulates a broad range of targets, such as GSK-3, NEDD4, iNOS, AMPAR, PSD95, Tau, NF-κB, CREB, and FKHR-L1 (8,9). Alternate Names serine/threonine protein kinase SGK; Serine/threonine-protein kinase Sgk1; serum/glucocorticoid regulated kinase 1; Serum/glucocorticoid-regulated kinase 1; SGK; SGK1; Sgk1 variant i3 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 45-60 Source/Isotype: Rabbit IgG