{"product_id":"cst-12120t","title":"CST,  12120T, Bcl-11B (D6F1) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying Bcl-11B. Validated for WB,IP,IF,IF,ChIP,ChIP. Available in 2 sizes. Highly specific and rigorously validated in-house, Bcl-11B (D6F1) Rabbit Monoclonal Antibody (CST #12120) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nFor optimal ChIP and ChIP-seq results, use 10 μl of antibody and 10 μg of chromatin (approximately 4 x 10 6 cells) per IP. This antibody has been validated using SimpleChIP ® Enzymatic Chromatin IP Kits.\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:100\nImmunofluorescence (Frozen): 1:100\nImmunofluorescence (Immunocytochemistry): 1:200\nChromatin IP: 1:50\nChromatin IP-seq: 1:50\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 16148 .\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Frozen), Immunofluorescence (Immunocytochemistry), Chromatin IP, Chromatin IP-seq\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nBcl-11B (D6F1) Rabbit Monoclonal Antibody recognizes endogenous levels of total Bcl-11B protein.\nSpecies Reactivity: Human, Mouse\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu391 of human Bcl-11B protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nBcl-11B (Ctip2) is a COUP-TF interacting protein that belongs to the C H type zinc finger protein family (1). Bcl-11B is highly expressed in the brain and is critical for the development of neurons, as well as other tissues and organs. Bcl-11B also plays an essential role in T cell lineage commitment and maintenance of T cell identity (1-3). Two isoforms of Bcl-11B are found to be encoded by the gene, possibly through exon-skipping (4). Bcl-11B is a transcription factor which binds to target genes through the 2 and 3 zinc-finger domains of exon 4 (3), while also interacting with various protein partners including COUP-TF proteins (1), the NuRD complex (5,6), HDAC1, HDAC2, and SUV39H1 (7). Research studies have shown that mutations and deletion of Bcl-11B contribute to the development of thymic lymphoma in mice and T cell acute lymphoblastic leukemia in humans, indicating a role as a tumor suppressor (4,8). Mechanistic studies have shown that Bcl-11B represses gene expression of the E3 ubiquitin ligase HDM2 in a p53-dependent manner (9).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nATL1; ATL1-alpha; ATL1-beta; ATL1-delta; ATL1-gamma; B cell CLL\/lymphoma 11B; B-cell CLL\/lymphoma 11B; B-cell CLL\/lymphoma 11B (zinc finger protein); B-cell CLL\/lymphoma 11B\/T-cell receptor delta constant region fusion protein; B-cell lymphoma\/leukaemia 11B; B-cell lymphoma\/leukemia 11B; BAF chromatin remodeling complex subunit BCL11B; BC11B; BCL-11B; BCL11B; BCL11B, BAF complex component; BCL11B\/TRDC fusion; COUP-TF-interacting protein 2; CTIP-2; CTIP2; hRit1; hRIT1-alpha; IDDFSTA; IMD49; Radiation-induced tumor suppressor gene 1 protein; RIT1; zinc finger protein hRit1 alpha; ZNF856B\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M\nSENSITIVITY: Endogenous\nMW (kDa): 120-130\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46797566345385,"sku":"12120T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-12120t","provider":"Iright","version":"1.0","type":"link"}